mProX™ Human DCLK2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1099	Magic™ Human DCAMKL2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

DCLK2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Lissencephaly and Band Heterotopia

Gene ID

166614

UniProt ID

Q8N568

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

A member of the doublecortin family and the protein kinase superfamily is encoded by this gene. The protein that this gene encodes has three main domains: a Cterminal serine/threonine protein kinase domain that resembles Ca2+/calmodulindependent protein kinase significantly, two Nterminal doublecortin domains that bind microtubules and control microtubule polymerization, and a serine/prolinerich domain that mediates multiple protein-protein interactions between the doublecortin and protein kinase domains. The encoded protein's ability to microtubule polymerize is unrelated to its protein kinase activity. According to mouse research, this gene, along with a family member called DCX, is involved in the creation of hippocampus organization. Their absence leads to a severe epileptic phenotype and death, which is similar to what is seen in lissencephaly patients who are human. The customized DCLK2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jason

Its reliability and user-friendly design have simplified the process of DCLK2 drug discovery in our lab. Aug 17 2023

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chat Laura

I have used the DCLK2 cell line extensively in my studies, and it consistently delivered outstanding performance. Jan 14 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 In vivo phenotypes of DCLK knockdown and knockout.

Neurons expressing DCLK1 shRNA showed decreased dendritic complexity, as revealed by sholl analysis and counting the number of terminal dendritic branches. Although the quantity of dendritic spines was only slightly elevated, the apical dendritic branches' spines were broader than those of the control dendrites.

Ref: Shin, Euikyung, et al. "Doublecortin-like kinase enhances dendritic remodelling and negatively regulates synapse maturation." Nature communications 4.1 (2013): 1440.

Pubmed: 23385585

DOI: 10.1038/ncomms2443

Research Highlights

Using numerous MTOCs, the yolk MT network of dclk2-GFP transgenic embryos can be utilized as a model to arrange microtubules in a spherical geometry.
Marsal, Maria, et al. "Multiple asters organize the yolk microtubule network during dclk2-GFP zebrafish epiboly." Scientific reports 12.1 (2022): 4072.
Pubmed: 35260695 DOI: 10.1038/s41598-022-07747-7

Based on the study's findings, DCLK2 may be a viable target for the therapy of metastatic breast cancer as it is implicated in EMT, cell invasion, and metastasis.
He, Yanling, et al. "Doublecortin-like kinase 2 promotes breast cancer cell invasion and metastasis." Clinical and Translational Oncology 25.4 (2023): 1102-1113.
Pubmed: 36477947 DOI: 10.1007/s12094-022-03018-z