mProX™ Human DCLK1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1098	Magic™ Human DCAMKL1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

DCLK1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Digestive and Renal Research

Related Diseases

Band Heterotopia and Zellweger Syndrome. Among its related pathways are B Cell Receptor Signaling Pathway and Development EGFR signaling pathway

Gene ID

9201

UniProt ID

O15075

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

A microtubule-associated protein kinase known as doublecortin-like kinase 1 (Dclk1) has been found to be a hallmark of tuft cells in the small intestine. It has been revealed that Dclk1 marks pancreatic and intestinal tumor stem cells. Recent data has established that a unique population of cells with stem cell characteristics and the capacity for self-renewal initiates and sustains most human cancers. More recently, it has been demonstrated that Dclk1+ cancer stem cells are essential for the initiation and spread of pancreatic and colon cancer. The customized DCLK1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Christopher

I recently purchased the DCLK1 cell line, and it has exceeded my expectations. Jun 14 2023

chat Verified Customer

chat Stephanie

We are delighted with the versatility and efficiency of DCLK1 cell line as a research tool. Feb 15 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 DCLK1-IN-1 inhibits DCLK1 phosphorylation and colony formation in RCC cell lines.

Immunoblots showing a significant reduction in DCLK1 serine 337 phosphorylation by DCLK1-IN-1 (10 µM) when applied to serum-starved RCC cells along with FBS for stimulation, with incubation at the specified times: 48 hours for ACHN, 24 hours for 786-O, and 48 hours for CAKI-1.

Ref: Ding, Ling, et al. "Inhibition of DCLK1 with DCLK1-IN-1 suppresses renal cell carcinoma invasion and stemness and promotes cytotoxic T-cell-mediated anti-tumor immunity." Cancers 13.22 (2021): 5729.

Pubmed: 34830884

DOI: 10.3390/cancers13225729

Research Highlights

A microtubule-associated protein containing a serine/threonine kinase domain at its C-terminus is called doublecortin like kinase protein 1 (Dclk1). Doublecortin and CaM kinase-like 1 protein (Dcamkl1), also known as KIAA0369, was initially identified as a radial glia cell marker in the context of microtubule polymerization and neuronal migration, perhaps playing a role in early neurogenesis.
Westphalen, C. Benedikt, Michael Quante, and Timothy C. Wang. "Functional implication of Dclk1 and Dclk1-expressing cells in cancer." Small GTPases 8.3 (2017): 164-171.
Pubmed: 27458755 DOI: 10.1080/21541248.2016.1208792

Through the activation of EMT, DCLK1 up-regulation may be a contributing factor to poor prognosis and metastasis of CRC. It's possible that DCLK1 can independently predict the prognosis for CRC.
Gao, Tianbo, et al. "DCLK1 is up-regulated and associated with metastasis and prognosis in colorectal cancer." Journal of cancer research and clinical oncology 142 (2016): 2131-2140.
Pubmed: 27520310 DOI: 10.1007/s00432-016-2218-0