mProX™ Human CSNK1A1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1082	Magic™ Human CK1α(CSNK1A1) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CSNK1A1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Digestive and Renal Research

Related Diseases

Enterobiasis and Amelogenesis Imperfecta, Type Iiia. Among its related pathways are CTNNB1 S45 mutants aren't phosphorylated and Regulation of activated PAK-2p34 by proteasome mediated degradation.

Gene ID

1452

UniProt ID

P48729

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

A member of the highly conserved serine/threonine protein kinase family is casein kinase 1, alpha 1 (CSNK1A1). There is expression of Csnk1a1 mRNA and protein in various mouse organs, including the ovary. Csnk1a1 mRNA and protein were found in preimplantation embryos following ovulation and fertilization, with two-cell stage embryos exhibiting the highest expression of both molecules. Preimplantation embryos' cytoplasm was likewise where the majority of the CSNK1A1 protein was found. Furthermore, the rate of blastocyst development was significantly reduced in zygotes upon Csnk1a1 knockdown. Moreover, embryonic developmental arrest was also induced by treating zygotes with the CSNK1A1-specific inhibitor D4476. These findings offer the first proof of CSNK1A1's unique role in the early stages of mouse embryonic development. The customized CSNK1A1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Kimberly

The CSNK1A1 KD cell line is easy to use and has become an essential tool in my lab. Dec 06 2020

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chat Gary

The CSNK1A1 KD cell line allowed me to perform rigorous experiments with consistent results. Jun 26 2022

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FAQ

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Published Data

Fig.1 CK1α mRNA and protein expression in MM patients and cell lines.

129 MM patients' (pts') PCs (left panel), 36 PCL patients' PCs (middle panel), and 18 MM cell lines' PCs (right panel) CSNK1A1 mRNA expression levels were compared to those of four healthy control PCs.

Ref: Manni, Sabrina, et al. "Inactivation of CK1α in multiple myeloma empowers drug cytotoxicity by affecting AKT and β-catenin survival signaling pathways." Oncotarget 8.9 (2017): 14604.

Pubmed: 28099937

DOI: 10.18632/oncotarget.14654

Research Highlights

The CSNK1A1-specific inhibitor D4476 also caused embryonic developmental arrest in zygotes treated with it. These findings offer the first proof of CSNK1A1's unique role in the early stages of mouse embryonic development.
Ma, Zengyou, et al. "Knockdown of Csnk1a1 results in preimplantation developmental arrest in mice." Theriogenology 198 (2023): 30-35.
Pubmed: 36542875 DOI: 10.1016/j.theriogenology.2022.12.018

These findings imply that inhibiting inflammatory factors through targeting Csnk1a1 has an anti-tumor effect, offering a novel approach to the treatment of gliomas.
Liu, Guanzheng, et al. "Csnk1a1 inhibition modulates the inflammatory secretome and enhances response to radiotherapy in glioma." Journal of Cellular and Molecular Medicine 25.15 (2021): 7395-7406.
Pubmed: 34216174 DOI: 10.1111/jcmm.16767