mProX™ Human CSK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1096	Magic™ Human CSK in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CSK

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Autoimmune Research

Related Diseases

Osteopetrosis and Lupus Erythematosus. Among its related pathways are Signaling downstream of RAS mutants and RAF/MAP kinase cascade.

Gene ID

1445

UniProt ID

P41240

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Kinase tyrosine-protein C-terminal is another name for CSK. The CSK gene in humans codes for the enzyme src kinase. Tyrosine residues in the C-terminal end of Src-family kinases (SFKs) are phosphorylated by this enzyme. The control of cell proliferation, differentiation, migration, and immunological response is significantly influenced by this non-receptor tyrosine-protein kinase. By phosphorylating members of the Src family of protein kinases at a conserved C-terminal tail location in Src, CSK inhibits the activity of the Src family of protein kinases. In order to block SFKs, CSK is first drawn to the plasma membrane by attaching itself to transmembrane proteins or adaptor proteins that are in close proximity to the membrane. By phosphorylating and keeping inactive a number of effector molecules, CSK then suppresses signaling through a variety of surface receptors. The customized CSK stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Donna

The CSK cell line has not disappointed. It consistently delivers accurate results, making my work much more impactful. May 08 2023

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chat Christopher

The protocol of CSK cell line was straightforward, and the results were consistent. Apr 21 2020

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FAQ

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Published Data

Fig.1 Comparison of the expression levels of CHK versus Csk in human neuroblastoma cell lines.

Comparative analysis using Western blotting of CHK and Csk expression levels in human neuroblastoma cell lines. Be(2)c cells and newly generated neuroblastoma cell lines, designated as 5425, 4030.2, 4181, and 9080.

Ref: Zagozdzon, Radoslaw, Yigong Fu, and Hava Karsenty Avraham. "Csk homologous kinase inhibits CXCL12-CXCR4 signaling in neuroblastoma." International journal of oncology 32.3 (2008): 619-623.

Pubmed: 18292939

Research Highlights

The Src-family protein tyrosine kinases (SFKs) are naturally inhibited by C-terminal Src kinase (CSK) and CSK-homologous kinase (CHK). Because constitutive activation of SFKs is linked to the development and spread of cancer, CSK and CHK regulate SFK activity in normal cells to keep it at a basal level.
Chong, Yuh-Ping, Terrence D. Mulhern, and Heung-Chin Cheng. "C-terminal Src kinase (CSK) and CSK-homologous kinase (CHK)-endogenous negative regulators of Src-family protein kinases: Mini Review." Growth factors 23.3 (2005): 233-244.
Pubmed: 16243715 DOI: 10.1080/08977190500178877

The essential enzymes for mammalian signal transduction are protein tyrosine kinases (PTK). Each PTK phosphorylates a single or small number of proteins on particular Tyr residues in order to maintain the integrity of signal transduction.
Lee, Sungsoo, et al. "Determination of the substrate-docking site of protein tyrosine kinase C-terminal Src kinase." Proceedings of the National Academy of Sciences 100.25 (2003): 14707-14712.
Pubmed: 14657361 DOI: 10.1073/pnas.2534493100