mProX™ Human CLEC6A Stable Cell Line
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- Membrane Protein Stable Cell Lines
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Published Data
Fig.1 Reduction in HIV-EBOV GP infection, irrespective of the presence of MBL, was observed upon shRNA-mediated dectin-2 (CLEC6A) knockdown.
Twenty-four candidate lectin, scavenger, and other putative receptors were targeted using pLKO.1 lentiviral vectors expressing 4 or 5 unique short hairpin RNA (shRNA) constructs per gene. HEK293F cells were transduced in quadruplicate with 4.6×10^8 viral particles (shRNA-expressing vectors or empty control vectors) and hexadimethrine bromide (6 µg/ml) at 37°C for 18 hours. Transduced cells were selected with 5 µg/ml puromycin over 48 hours. Subsequently, cells were infected in quadruplicate with HIV-EBOV GP virion-like particles (1000 pg p24/100 µl) in the presence or absence of rhMBL. After 48 hours, rates of single-round infection (luciferase assay) were measured, and the results were adjusted for cell viability. The percentage change in infection was normalized to the empty pLK0.1 control vector (CTRL).
Ref: Brudner, Matthew, et al. "Lectin-dependent enhancement of Ebola virus infection via soluble and transmembrane C-type lectin receptors." PloS one 8.4 (2013): e60838.
Pubmed: 23573288
DOI: 10.1371/journal.pone.0060838
Research Highlights
Wang, Jie. et al. "Integration of Non-Coding RNA and mRNA Profiles Reveals the Mechanisms of Rumen Development Induced by Different Types of Diet in Calves." Genes, 2023.
In intensive dairy farming, selecting appropriate feed types and understanding gastrointestinal digestive mechanisms is crucial for the wellbeing and growth of calves. However, the impact of changing the molecular genetic basis and regulatory mechanism using different feed types on rumen development remains unclear. To address this, a study was conducted using nine Holstein bull calves, randomly allocated to three diet groups: GF (concentrate), GFF (alfalfa:oat grass = 3:2), and TMR (concentrate:alfalfa grass:oat grass:water = 0.30:0.12:0.08:0.50). After 80 days, rumen tissue and serum samples were collected for physiological and transcriptomic analysis. The results showed that the TMR group exhibited higher serum α-amylase and ceruloplasmin activity compared to the other groups. Furthermore, the TMR diet significantly enriched the pathways of rumen epithelial development and stimulated rumen cell growth, including the Hippo signaling pathway, Wnt signaling pathway, thyroid hormone signaling pathway, and absorption of protein and fat. The constructed circRNA/lncRNA-miRNA-mRNA networks, including novel_circ_0002471, novel_circ_0012104, TCONS_00946152, TCONS_00960915, bta-miR-11975, bta-miR-2890, PADI3, and CLEC6A, were found to participate in metabolic pathways related to lipid, immune system, oxidative stress, and muscle development. In summary, the TMR diet was found to improve rumen digestive enzyme activities, nutrient absorption, and the expression of differentially expressed genes associated with energy homeostasis and microenvironment balance. Therefore, it can be concluded that the TMR diet is more beneficial for promoting rumen growth and development compared to the GF and GFF diets.
Wang, Jie. et al. "Integration of Non-Coding RNA and mRNA Profiles Reveals the Mechanisms of Rumen Development Induced by Different Types of Diet in Calves." Genes, 2023.
Pubmed:
37239453
DOI:
10.3390/genes14051093
S Reilly, Cavan. et al. "Investigation of Causal Effects of Protein Biomarkers on Cardiovascular Disease in Persons With HIV." The Journal of infectious diseases, 2023.
The association between individuals with HIV (PWH) and an increased risk for cardiovascular disease (CVD) is not fully understood. A study was conducted to determine if a set of biomarkers had a significant correlation with the occurrence of CVD among PWH. Through the use of Mendelian randomization (MR), potential causal associations were identified. The results of this research may lead to a better understanding of the relationship between HIV and CVD, ultimately aiding in the development of preventative and treatment strategies.
S Reilly, Cavan. et al. "Investigation of Causal Effects of Protein Biomarkers on Cardiovascular Disease in Persons With HIV." The Journal of infectious diseases, 2023.
Pubmed:
36580481
DOI:
10.1093/infdis/jiac496
