mProX™ Human CLEC4C Stable Cell Line

Product Information

Target Family

Immune Checkpoint

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;Jurkat

Target Classification

Immune Checkpoint Cell Lines

Target Research Area

Immunology Research

Related Diseases

Cutaneous Lupus Erythematosus; Cardiomyopathy, Dilated, 1C, With Or Without Left Ventricular Noncompaction

Gene ID

Human:170482

UniProt ID

Human:Q8WTT0

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The application of CLEC4C, a gene, has been studied in various contexts. In the context of juvenile idiopathic arthritis (JIA), CLEC4C was identified as one of the key genes associated with the disease. It exhibited high expression in B cells, suggesting its potential role in the pathogenesis of JIA. In the context of COVID-19, CLEC4C was found to be associated with the risk of short-term clinical relapse in patients with Crohn's disease who stopped infliximab treatment. Additionally, CLEC4C was identified as a putative causal expression quantitative trait locus (eQTL) in whole blood RNA-seq data from COVID-19 infected individuals. These findings suggest that CLEC4C may have prognostic value and potential implications for the treatment and investigation of JIA, Crohn's disease, and COVID-19.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Morgan Smith (Verified Customer)

What is the significance of CLEC4C in heart transplantation? Dec 06 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

Pre-transplantation circulating levels of CLEC4C, a marker of plasmacytoid dendritic cells, may identify heart transplant recipients at risk for primary graft dysfunction, suggesting its role in transplant immunology. Dec 06 2021

chat Cameron Williams (Verified Customer)

How does CLEC4C expression relate to immune cell quantification? Apr 13 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

Measuring CLEC4C gene expression provides an alternative method for quantifying plasmacytoid dendritic cell numbers in human samples, aiding in immune cell profiling. Apr 13 2022

Published Data

Fig.1 The dynamics of intracellular calcium mobilization within transfected Jurkat cells, denoting transgene expression atop, upon stimulation through crosslinking (indicated by the arrow) with either control (IgG1) or anti-BDCA2 monoclonal antibody (mAb), were investigated.

The activation cascade involving calcium influx, a crucial event in cellular response following ITAM signaling, was efficiently initiated upon BDCA2 crosslinking in Jurkat cells co-transfected with both BDCA2 and FcεRIγ. In contrast, cells transfected with BDCA2 alone failed to elicit this response, thus reaffirming the essential role of FcεRIγ in facilitating BDCA2 signaling.

Ref: Cao, Wei, et al. "BDCA2/FcεRIγ complex signals through a novel BCR-like pathway in human plasmacytoid dendritic cells." PLoS biology 5.10 (2007): e248.

Pubmed: 17850179

DOI: 10.1371/journal.pbio.0050248

Research Highlights

Zhang, Wenbo. et al. "Immune Cell-Related Genes in Juvenile Idiopathic Arthritis Identified Using Transcriptomic and Single-Cell Sequencing Data." International journal of molecular sciences, 2023.
Juvenile idiopathic arthritis (JIA) represents the most prevalent chronic rheumatic ailment affecting children. Researchers have delved into the disease's heterogeneity using single-cell RNA sequencing (scRNA-seq), aiming to bridge existing gaps in knowledge. Their exploration unveiled significant differences in five immune cell types-plasma cells, naive CD4 T cells, memory-activated CD4 T cells, eosinophils, and neutrophils-between normal control (NC) and JIA samples. Subsequently, they identified 168 differentially expressed immune cell-related genes (DE-ICRGs) through a convergence of 13,706 genes uncovered by WGCNA and 286 differentially expressed genes (DEGs) distinguishing JIA from NC specimens. Further analysis pinpointed four pivotal genes-SOCS3, JUN, CLEC4C, and NFKBIA-via a protein-protein interaction (PPI) network and three machine learning algorithms. Functional enrichment results linked SOCS3, JUN, and NFKBIA to hallmark TNF-α signaling via NF-κB. In addition, single-cell data analysis clustered cells from JIA samples into four distinct groups-B cells, monocytes, NK cells, and T cells. Among these, CLEC4C and JUN exhibited the highest expression in B cells, while NFKBIA and SOCS3 displayed peak expression in monocytes. Finally, real-time quantitative PCR (RT-qPCR) confirmed the expression patterns of these three key genes, suggesting their potential prognostic significance in JIA, with far-reaching implications for its treatment and research.
Zhang, Wenbo. et al. "Immune Cell-Related Genes in Juvenile Idiopathic Arthritis Identified Using Transcriptomic and Single-Cell Sequencing Data." International journal of molecular sciences, 2023.
Pubmed: 37445800 DOI: 10.3390/ijms241310619

Yazici, Duygu. et al. "Disrupted epithelial permeability as a predictor of severe COVID-19 development." Allergy, 2023.
The impaired integrity of the epithelial barrier in the gastrointestinal tract is a significant factor in the onset of various inflammatory diseases. Therefore, the potential of biomarkers indicating epithelial barrier dysfunction was examined for its ability to predict the severity of COVID-19.
Yazici, Duygu. et al. "Disrupted epithelial permeability as a predictor of severe COVID-19 development." Allergy, 2023.
Pubmed: 37422701 DOI: 10.1111/all.15800