mProX™ Human CLCN2 Stable Cell Line

Product Information

Target Protein

CLCN2

Target Family

Voltage Gated Chloride Channel

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Hyperaldosteronism; Leukoencephalopathy With Ataxia

Gene ID

1181

UniProt ID

P51788

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The CLCN2 gene is a member of the CLC gene family, which codes for the production of chloride channels. The instructions needed to create the ClC-2 chloride channel are encoded by the CLCN2 gene. These channels, which move chloride ions in and out of cells, are embedded in the outer membrane of the majority of cells. It is believed that the channel plays a particularly significant role in brain neurons, which are nerve cells. The ClC-2 channel contributes to the uptake and release of water and preserves the proper ion balance inside cells, which in turn controls the size (volume) of neurons. The customized CLCN2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Rebecca

The CLCN2 cell line is a good product for general routine use from Creative Biolabs. Jul 30 2021

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I recently purchased the CLCN2 cell line, and it has been a valuable tool in my laboratory work. The protocol provided by Creative Biolabs is straightforward, and I achieved consistent results. Jul 28 2022

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FAQ

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Published Data

Fig.1 Sertoli cell-specific Clcn2 disruption causes testicular degeneration in mice.

Membrane fractions obtained from the whole testis of adult Clcn2lox/lox, GC-ΔC2 (Clcn2 knockout specific to germ cells), Clcn2-/-, and SC-ΔC2 animals were subjected to Western blot examination of ClC-2. The BCA assay was used to determine the protein loading levels.

Ref: Göppner, Corinna, et al. "Cellular basis of ClC-2 Cl− channel-related brain and testis pathologies." Journal of Biological Chemistry 296 (2021).

Pubmed: 33187987

DOI: 10.1074/jbc.RA120.016031

Research Highlights

These results reveal the involvement of anion channels in the determination of the glomerulosa membrane potential, aldosterone production, and hypertension for the first time. They determine the etiology of a significant portion of primary aldosteronism with early onset.
Scholl, Ute I., et al. "CLCN2 chloride channel mutations in familial hyperaldosteronism type II." Nature genetics 50.3 (2018): 349-354.
Pubmed: 29403011 DOI: 10.1038/s41588-018-0048-5

The most prevalent and treatable type of secondary arterial hypertension is primary aldosteronism. Hyperpolarization-activated Cl-currents were seen in patch-clamp examination of glomerulosa cells in mouse adrenal gland slices; these currents were eliminated in Clcn2-/-mice.
Fernandes-Rosa, Fabio L., et al. "A gain-of-function mutation in the CLCN2 chloride channel gene causes primary aldosteronism." Nature genetics 50.3 (2018): 355-361.
Pubmed: 29403012 DOI: 10.1038/s41588-018-0053-8