mProX™ Human CHUK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1168	Magic™ Human IKK-alpha in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CHUK

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

H1437; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Fetal Encasement Syndrome and Bartsocas-Papas Syndrome 2. Among its related pathways are MyD88 dependent cascade initiated on endosome and TNFR1 Pathway

Gene ID

1147

UniProt ID

O15111

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Nuclear factor inhibitor The CHUK gene in humans encodes kappa-B kinase subunit alpha (IKK-α), which is sometimes referred to as IKK1 or conserved helix-loop-helix ubiquitous kinase (CHUK). The IκB kinase complex, which includes IKK-α, is crucial for controlling the NF-κB transcription factor. Nevertheless, IKK-α is believed to regulate epidermal differentiation independently of the NF-κB pathway and has numerous other cellular targets. The potential therapeutic benefit of inhibiting IκB kinase (IKK) and IKK-related kinases, such as IKBKE (IKKε) and TANK-binding kinase 1 (TBK1), has been explored for the management of cancer and inflammatory disorders. The customized CHUK stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat James

I've been using the mouse CHUK overexpression cell line for my studies. May 19 2021

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The CHUK overexpression cell line showed good performance in our hands. Feb 20 2020

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FAQ

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Published Data

Fig.1 Generation of IKKα knockdown human NSCLC cell lines.

In each of the human NSCLC lines stably transduced with the Dox-inducible IKKα shRNA lentiviral vector, Western blots demonstrate the loss of IKKα protein expression in response to Dox treatment. In contrast, IKKα protein levels remain wild type (WT) in the same NSCLC lines stably transduced with an empty lentiviral vector control lacking the IKKα shRNA insert (IKKαWT-lentiviral vector controls).

Ref: Chavdoula, Evangelia, et al. "CHUK/IKK-α loss in lung epithelial cells enhances NSCLC growth associated with HIF up-regulation." Life science alliance 2.6 (2019).

Pubmed: 31792060

DOI: 10.26508/lsa.201900460

Research Highlights

The phrase CHUK plays a role in the evolution of IS. Patients with IS may have changes in blood pressure and cholesterol levels due to CHUK mutations rs2230804 and rs3808917. The polymorphisms for CHUK rs3808916, rs2230804, and rs3808917, however, are not linked to an increased risk of IS.
Huang, Jingyan, et al. "Association of CHUK gene polymorphism and ischemic stroke in the Han Chinese population." Journal of Clinical Neuroscience 88 (2021): 271-276.
Pubmed: 33992196 DOI: 10.1016/j.jocn.2021.04.009

The aim of the study was to examine the potential anti-cancer effects and underlying molecular mechanisms of cytostasis induced by the anti-microtubule medication ABT-751 in two genetically divergent urinary bladder urothelial carcinoma (UBUC)-derived cell lines, BFTC905 and J82.
Dehghanian, Seyedeh Zahra, et al. "ABT-751 induces multiple anticancer effects in urinary bladder urothelial carcinoma-derived cells: Highlighting the induction of cytostasis through the inhibition of SKP2 at both transcriptional and post-translational levels." International journal of molecular sciences 22.2 (2021): 945.
Pubmed: 33478005 DOI: 10.3390/ijms22020945