mProX™ Human CHRNA7/ric3 Stable Cell Line

Product Information

Target Protein

CHRNA7/ric3

Target Family

Acetylcholine Nicotinic Channel

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Epilepsy

Gene ID

1139

UniProt ID

P36544

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Initially, a P50 auditory-evoked potential deficit-a neurophysiological characteristic frequently observed in schizophrenia-was associated with the alpha7 nicotinic receptor (CHRNA7). The genetic marker is found at chromosome 15q13-14, a linkage that results from nine European-American high-density psychotic families that are impacted many times. Subsequent analysis and validation of the findings from the previous inquiry revealed a significant genotype-wise disequilibrium at D15S165. This polymorphic simple sequence marker is located 120 kb away from the coding area of CHRNA7. The human gene is a portion of a duplicate cassette of expressed sequences that are almost next to one another. Four new exons and six exons that replicate exons 5-10 of the full-length CHRNA7 make up the expressed sequence. The customized CHRNA7/ric3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Deborah

The performance of the CHRNA7/ric3 cell line was consistent, and the company's support team was responsive and helpful throughout the process. Sep 19 2021

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chat James

I have tested the CHRNA7/ric3 cell line from Creative Biolabs as one of the most reliable and versatile options available. Mar 18 2023

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FAQ

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Published Data

Fig.1 Expression of CHRNA7 and CHRFAM7A during neuronal differentiation of induced pluripotent stem cells (iPSCs).

quantitative polymerase chain reaction study of reverse transcription-expressed CHRFAM7A and CHRNA at three different stages of neural differentiation: D0 (pluripotentiPSC), D25 (MGE progenitors), and D40 (neurons). Using primers, the distinct breakpoint sequence is amplified and the CHRFAM7A and CHRNA7 mRNAs are distinguished.

Ref: Ihnatovych, Ivanna, et al. "iPSC model of CHRFAM7A effect on α7 nicotinic acetylcholine receptor function in the human context." Translational Psychiatry 9.1 (2019): 59.

Pubmed: 30710073

DOI: 10.1038/s41398-019-0375-z

Research Highlights

The gene for the human α7 neuronal nicotinic acetylcholine receptor (CHRNA7) is widely expressed in the peripheral and central nervous systems. Numerous conditions involving cognitive abnormalities, such as schizophrenia, bipolar disorder, ADHD, epilepsy, Alzheimer's disease, and Rett syndrome, are genetically associated with CHRNA7.
Sinkus, Melissa L., et al. "The human CHRNA7 and CHRFAM7A genes: A review of the genetics, regulation, and function." Neuropharmacology 96 (2015): 274-288.
Pubmed: 25701707 DOI: 10.1016/j.neuropharm.2015.02.006

The CHRNA7 gene encodes the alpha7 nicotinic acetylcholine receptor, which is thought to be a possible target for therapeutic intervention. This receptor has been linked to a number of psychiatric and behavioral disorders, including autism, bipolar disorder, schizophrenia, epilepsy, Alzheimer's disease, and Parkinson's disease.
Yin, Jiani, et al. "Chrna7 deficient mice manifest no consistent neuropsychiatric and behavioral phenotypes." Scientific reports 7.1 (2017): 39941.
Pubmed: 28045139 DOI: 10.1038/srep39941