mProX™ Human CHRNA4/CHRNB2 Stable Cell Line

Product Information

Target Protein

CHRNA4/CHRNB2

Target Family

Acetylcholine Nicotinic Channel

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Epilepsy

Gene ID

1137

UniProt ID

P43681

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

In roughly 12-15% of ADNFLE patients with a strong family history, the clinical phenotype is caused by the nAChR subunit genes CHRNA4 and CHRNB2. The brains of mammals express both genes, and the proteins they encode combine to form one of the most commonly expressed nAChRs (3α4/2β2 or 2α4/3β2). Given that mutations in these genes induce a seizure phenomenology that begins in the frontal lobe and seldom demonstrates subsequent generalization, the widespread expression pattern of this nAChR subtype is unexpected. The pathomechanisms preventing CHRNA4 and CHRNB2 mutations from having a more widespread effect are still just speculative. The customized CHRNA4/CHRNB2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Sandra

Its reliability and user-friendly design have simplified the process of CHRNA4/CHRNB2 drug discovery in our lab. Sep 04 2021

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chat Karen

I have used the CHRNA4/CHRNB2 cell line extensively in my studies, and it consistently delivered outstanding performance. Jan 12 2023

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FAQ

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Published Data

Fig.1 Whole-cell current traces elicited by nicotine at −70 mV.

HEK cells expressing a4β2 (WT) nAChRs exhibit representative whole-cell current traces when exposed to the appropriate dose of nicotine.

Ref: Cadieux-Dion, Maxime, et al. "Variants in CHRNB2 and CHRNA4 identified in patients with insular epilepsy." Canadian Journal of Neurological Sciences 47.6 (2020): 800-809.

Pubmed: 32536355

DOI: 10.1017/cjn.2020.126

Research Highlights

The purpose of this study is to look into CHRNB2, CHRNA2, and CHRNA4 gene mutations in the Chinese population that has autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE).
Chen, Zhihong, et al. "Mutational analysis of CHRNB2, CHRNA2 and CHRNA4 genes in Chinese population with autosomal dominant nocturnal frontal lobe epilepsy." International Journal of Clinical and Experimental Medicine 8.6 (2015): 9063.
Pubmed: 26309560

The primary goal of the current study was to determine if patients from smoker support programs' responses to smoking cessation medications are correlated with the CHRNA4 and CHRNB2 polymorphisms in the context of personalized medicine.
Rocha Santos, Juliana, et al. "CHRNA4 rs1044396 is associated with smoking cessation in varenicline therapy." Frontiers in genetics 6 (2015): 46.
Pubmed: 25774163 DOI: 10.3389/fgene.2015.00046