mProX™ Human CHRNA4/CHRNA6/CHRNB2 Stable Cell Line

Product Information

Target Protein

CHRNA4/CHRNA6/CHRNB2

Target Family

Acetylcholine Nicotinic Channel

Target Protein Species

Human

Host Cell Type

STC-1; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Epilepsy

Gene ID

1137

UniProt ID

P43681

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Receptor polypeptides known as nicotinic acetylcholine receptors, or nAChRs, react to the neurotransmitter acetylcholine. Substances like the agonist nicotine also have an effect on nicotinic receptors. They are present in many different tissues of numerous creatures, including muscle and the peripheral and central nervous systems. They are the main receptor in the muscle at the neuromuscular junction, where motor neuron and muscle communicate to control muscle contraction. They serve two purposes in the peripheral nervous system: (1) they are the receptors on skeletal muscle that receive the release of acetylcholine, which signals the contraction of muscles; and (2) they are the outgoing signals from the presynaptic to the postsynaptic cells within the sympathetic and parasympathetic nervous systems. nAChRs control inflammatory processes in the immune system and communicate via unique intracellular pathways. The customized CHRNA4/CHRNA6/CHRNB2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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I recently purchased the CHRNA4/CHRNA6/CHRNB2 cell line, and it has exceeded my expectations. Jan 29 2023

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We are delighted with the versatility and efficiency of CHRNA4/CHRNA6/CHRNB2 cell line as a research tool. Sep 24 2022

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FAQ

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Published Data

Fig.1 Expression of nAChR subunits, taste receptors, and BDNF in STC-1 cells.

Based on the published sequences, consensus primers were constructed to amplify the mouse nAChR subunits, taste receptor (T2R38), downstream signaling intermediate (TRPM5), and BDNF. The mRNAs for the nAChR subunits, chrna3, chrna4, chrna5, chrna6, chrna7, chrnb2, and chrnb4, were found in the STC-1 cell cDNA sample, according to the expected sizes of the PCR results.

Ref: Qian, Jie, et al. "Nicotine-induced effects on nicotinic acetylcholine receptors (nAChRs), Ca2+ and brain-derived neurotrophic factor (BDNF) in STC-1 cells." PLoS One 11.11 (2016): e0166565.

Pubmed: 27846263

DOI: 10.1371/journal.pone.0166565

Research Highlights

By employing standards for smoking behavior that go beyond the one-cigarette-per-day item, a prevalent CHRNA4 variation with significant regulatory characteristics that support nicotine dependency and the negative effects of smoking was found.
Hancock, D. B., et al. "Genome-wide meta-analysis reveals common splice site acceptor variant in CHRNA4 associated with nicotine dependence." Translational psychiatry 5.10 (2015): e651-e651.
Pubmed: 26440539 DOI: 10.1038/tp.2015.149

The nicotinic system is linked to a number of neuropsychiatric disorders and is crucial for cognitive regulation. Nevertheless, little is known about how genetic diversity in this system affects an individual's capacity for cognitive regulation, and the brain mechanisms behind these genetic contributions are still mostly unknown.
Sadaghiani, Sepideh, et al. "Overdominant effect of a CHRNA4 polymorphism on cingulo-opercular network activity and cognitive control." Journal of Neuroscience 37.40 (2017): 9657-9666.
Pubmed: 28877969 DOI: 10.1523/JNEUROSCI.0991-17.2017