mProX™ Human CHRNA3/CHRNB4 Stable Cell Line

Product Information

Target Protein

CHRNA3/CHRNB4

Target Family

Acetylcholine Nicotinic Channel

Target Protein Species

Human

Host Cell Type

HBO; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Bladder Dysfunction; Autonomic

Gene ID

1136

UniProt ID

P32297

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The nAChR gene cluster, located on chromosome 15q25.1, is a member of the ligand-gated ion channel superfamily. It is widely distributed in the brain and mediates fast cholinergic synaptic transmission by binding the ligands nicotine and acetylcholine. This process also involves activating downstream signaling networks that support angiogenesis, migration, and cell proliferation. Additionally, nAChRs have been linked to smoking habits, nicotine dependence, and an increased risk of lung cancer and chronic obstructive pulmonary disease (COPD). The customized CHRNA3/CHRNB4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The convenience of the CHRNA3/CHRNB4 cell line has significantly streamlined my workflow. Jul 28 2020

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The CHRNA3/CHRNB4 cell line is of superior quality, and I am confident in its reliability. Jan 19 2020

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FAQ

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Published Data

Fig.1 Expression of CHRN subunits, taste receptors, and intracellular signaling intermediates in HBO and HEK293 cells.

Based on the published sequences, consensus primers were constructed to amplify GPCRs, downstream signaling intermediates, and CHRN subunits. Using particular AChRα4 and AChRα5 antibodies, α4 and α5 protein expression was found in HBO and HEK293 cell lysates by Western blot analysis.

Ref: Qian, Jie, et al. "Nicotinic acetylcholine receptor (CHRN) expression and function in cultured human adult fungiform (HBO) taste cells." PLoS One 13.3 (2018): e0194089.

Pubmed: 29513745

DOI: 10.1371/journal.pone.0194089

Research Highlights

In GWAS (COGEND, UW-TTURC, SAGE), haplotype/diplotype analysis of rs880395 and rs1948 plus rs16969968 (a nonsynonymous CHRNA5 risk variable) reveals a nicotine dependency risk profile that is only partially explained by rs16969968 alone. This nicotinic regulome, an illustration of a small gene cluster, is regulated by intricate genetic variation, which has significant ramifications for the interpretation of GWAS.
Barrie, Elizabeth S., et al. "The CHRNA5/CHRNA3/CHRNB4 nicotinic receptor regulome: genomic architecture, regulatory variants, and clinical associations." Human mutation 38.1 (2017): 112-119.
Pubmed: 27758088 DOI: 10.1002/humu.23135

The CHRNA5/A3/B4 gene cluster in the 15q25 area has three polymorphisms: rs1051730, rs16969968, and rs8034191. These polymorphisms were linked to LC risk, which may be impacted by smoking status and ethnicity.
Yi, Xingxu, et al. "The relationship between CHRNA5/A3/B4 gene cluster polymorphisms and lung cancer risk: An updated meta-analysis and systematic review." Medicine 100.6 (2021).
Pubmed: 33578531 DOI: 10.1097/MD.0000000000024355