mProX™ Human CHRNA1/CHRNB1/CHRND/CHRNE Stable Cell Line

Product Information

Target Protein

CHRNA1/CHRNB1/CHRND/CHRNE

Target Family

Acetylcholine Nicotinic Channel

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research

Related Diseases

Bladder Dysfunction; Autonomic

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The CHRNA1 gene in humans encodes the protein known as neuronal acetylcholine receptor subunit alpha-1, or nAChRα1. Some nicotinic acetylcholine receptors (nAchR) contain a protein that is encoded by this gene. The cholinergic receptor in muscles is made up of five subunits, two of which are alpha isoforms and one of each of beta, gamma, and delta subunits.2. An alpha subunit involved in acetylcholine binding and channel gating is encoded by this gene. Different isoforms are encoded by alternatively spliced transcript variants that have been discovered. The customized CHRNA1/CHRNB1/CHRND/CHRNE stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The CHRNA1/CHRNB1/CHRND/CHRNE cell line has not disappointed. It consistently delivers accurate results, making my work much more impactful. Aug 30 2022

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The protocol of CHRNA1/CHRNB1/CHRND/CHRNE cell line was straightforward, and the results were consistent. Nov 21 2020

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FAQ

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Published Data

Fig.1 Stable expression of AChR subunits and rapsyn in HEK293T cells.

Using Western blotting, real-time PCR, and immunofluorescence, the expression of clustered AChR in KL525 cells was confirmed. The coexpressed AChR α1 & δ subunits in KL525 cells showed a modest decreasing tendency at passages 1, 5, and 15, according to flow cytometric analysis, but there was no statistically significant difference seen, suggesting a stable expression of clustered AChR.

Ref: Cai, Yu, et al. "A stable cell line expressing clustered AChR: a novel cell-based assay for Anti-AChR antibody detection in Myasthenia Gravis." Frontiers in Immunology 12 (2021): 666046.

Pubmed: 34305897

DOI: 10.3389/fimmu.2021.666046

Research Highlights

(CHRNA1, CHRNB1, CHRND, and CHRNE) or proteins like MUSK, RAPSN, or DOK7 that are crucial for preserving the form or functionality of the NMJ. 3 Acetylcholine receptor (AChR) defects can be either primary, arising from a reduction in the expression of one of the subunits, or they can be functionally separated into two groups: slow-channel (SCCMS) and fast-channel (FCCMS) congenital myasthenic syndromes.
Neto, Osorio Abath, et al. "Nonlethal CHRNA1-related congenital myasthenic syndrome with a homozygous null mutation." Canadian journal of neurological sciences 44.1 (2017): 125-127.
Pubmed: 27748205 DOI: 10.1017/cjn.2016.322

Elderly people's quality of life is significantly impacted by sarcopenia, yet its exact molecular cause is still unknown. Muscle atrophy and age-related mobility problems are associated with degeneration of muscle innervation. In aging rodents and the skeletal muscle of the elderly, CHRNA1 expression is elevated.
Liao, Zhiyin, et al. "CHRNA1 induces sarcopenia through neuromuscular synaptic elimination." Experimental Gerontology 166 (2022): 111891.
Pubmed: 35809807 DOI: 10.1016/j.exger.2022.111891