mProX™ Human CHRM5 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Made to Order Inquiry
InquiryProduct Information
Product Properties
Protocols
Please visit our protocols page.
Customer Reviews
There are currently no Customer reviews or questions for mProX™ Human CHRM5 Stable Cell Line (S01YF-0923-PY131). Click the button above to contact us or submit your feedback about this product.
Michelle (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Carol (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Published Data
Fig.1 The IP1 accumulation of the Gln184Arg (CHRM5) variant is indistinguish-able from the WT M5mAChR
IP1 accumulation after ACh activation of the Gln184Arg mutant is identical to the response found at the WT M5mAChR. Data indicate the mean S.E.M. of three separate experiments done in duplicate for all experiments.
Ref: Schneider, Sophia, et al. "Recessive CHRM5 variant as a potential cause of neurogenic bladder." American Journal of Medical Genetics Part A (2023).
Pubmed: 37213061
DOI: 10.1002/ajmg.a.63241
Research Highlights
Schneider S, et al. "Recessive CHRM5 variant as a potential cause of neurogenic bladder.." American journal of medical genetics. Part A, 2023.
Neurogenic bladder results from a disruption in the neuronal pathways that regulate bladder relaxation and contraction. In severe cases, this condition can cause complications such as vesicoureteral reflux, hydroureter, and chronic kidney disease, which overlap with congenital anomalies of the kidney and urinary tract (CAKUT). To identify potential new genetic causes of neurogenic bladder, the authors used exome sequencing on a group of families with CAKUT. Through this process, they discovered a homozygous missense variant (p.Gln184Arg) in CHRM5, a gene that codes for a muscarinic acetylcholine receptor and has been linked to bladder overactivity in mice. Despite similarities to another cholinergic bladder neuron receptor (CHRNA3) known to cause neurogenic bladder, further in vitro studies are needed to determine the gene's candidacy. Additional discovery of CHRM5 variants in other families could provide further evidence for its role in neurogenic bladder with secondary CAKUT complications.
Pubmed:
37213061
DOI:
10.1002/ajmg.a.63241
Vinay CM, et al. "Integrated LC-MS/MS and network pharmacology approach for predictingactive ." Journal of biomolecular structure & dynamics, 2023.
Tribulus terrestris L. (Gokshura) is a widely used medicinal herb known for its potential in treating various diseases, including cardiac diseases. Despite its extensive use, the active ingredients and mechanism of action for treating cardiac diseases have not been fully elucidated. To address this, researchers designed a study using an integrated approach of metabolomics and network pharmacology. Putative compounds were identified through HPLC-QTOF-MS/MS analysis and potential key targets and pathways were predicted through network pharmacology analysis. In silico molecular docking and simulation resulted in the identification of several active ingredients with potential to treat cardiac diseases. Further in vivo and in vitro studies are recommended to confirm the efficacy of these compounds.
Pubmed:
37042962
DOI:
10.1080/07391102.2023.2199076
