mProX™ Human CHEK1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1080	Magic™ Human CHK1(CHEK1) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CHEK1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HCC; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Ataxia-Telangiectasia and Li-Fraumeni Syndrome. Among its related pathways are Homology Directed Repair and Regulation of activated PAK-2p34 by proteasome mediated degradation

Gene ID

1111

UniProt ID

O14757

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Chk1, also known as checkpoint kinase 1, is a serine/threonine-specific protein kinase that is encoded by the CHEK1 gene in humans. The cell cycle checkpoint response and the DNA damage response (DDR) are coordinated by Chk1. In order to stop injured cells from advancing through the cell cycle, Chk1 activation triggers the start of cell cycle checkpoints, cell cycle arrest, DNA repair, and cell death. In addition to being a crucial regulator of the cell cycle and cell survival, Chk1 is a fundamental part of genome surveillance pathways. In addition to being necessary for the start of DNA damage checkpoints, Chk1 has also been demonstrated to be involved in the regular (unperturbed) cell cycle. Chk1 affects the S phase, G2/M transition, and M phases of the cell cycle. The customized CHEK1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Sharon

This mouse CHEK1 cell line enabled us to conduct our experiments with confidence, and we achieved publication-worthy data. Sep 01 2021

chat Verified Customer

chat Ronald

The CHEK1 cell line demonstrated remarkable specificity and sensitivity, which helped us unravel complex signaling pathways. Oct 14 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 ATR and CHK1 are potential therapeutic targets for HCC.

In the GSE14520 cohort and the TCGA database, tumor tissues had higher levels of ATR and CHEK1 mRNA than non-tumor tissues. Moreover, patients with the highest levels of ATR or CHEK1 mRNA in their tumors had the lowest survival rates in a TCGA cohort of 365 HCC patients categorized by tertile cut-off values.

Ref: Guo, Yuchen, et al. "Targeting CDC7 potentiates ATR-CHK1 signaling inhibition through induction of DNA replication stress in liver cancer." Genome Medicine 13.1 (2021): 1-15.

Pubmed: 34663432

DOI: 10.1186/s13073-021-00981-0

Research Highlights

The potential involvement of CHEK1 in solid tumors suggests that it could be a target for cancer treatment intervention. Additional research on the relationship between CHEK1 and other co-expressed genes may provide light on additional ways that this gene is regulated in cancer patients.
Fadaka, Adewale Oluwaseun, et al. "Gene expression alterations and molecular analysis of CHEK1 in solid tumors." Cancers 12.3 (2020): 662.
Pubmed: 32178478 DOI: 10.3390/cancers12030662

One type of malignant cancer that poses a major risk to women's health is breast cancer (BC). Scientists studying BC have discovered that it results from a combination of internal genetic alterations and external environmental factors. A critical cell cycle speed limit is cell cycle checkpoint kinase 1 (CHEK1).
Wu, Mei, et al. "The clinical significance of CHEK1 in breast cancer: a high-throughput data analysis and immunohistochemical study." International journal of clinical and experimental pathology 12.1 (2019): 1.
Pubmed: 31933717 DOI: PMC6944032