mProX™ Human CDK9 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1077	Magic™ Human CDK9/CycT1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDK9

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research; Cardiovascular Research; Auditory and Otology Research; Ocular Research; Reproductive Research

Related Diseases

Hypothyroidism, Congenital, Nongoitrous, 6 and Charge Syndrome. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and HIV Life Cycle

Gene ID

1025

UniProt ID

P50750

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

A cyclin-dependent kinase linked to P-TEFb is called cyclin-dependent kinase 9, or CDK9. This gene codes for a protein that is a member of the CDK (cyclin-dependent kinase) family. Members of the CDK family are well-known cell cycle regulators and bear a striking resemblance to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It was discovered that this kinase is a part of the multiprotein complex TAK/P-TEFb. Cyclin T or Cyclin K, this protein's regulatory subunit, forms a complex with it and controls it. It was discovered that the HIV-1 Tat protein interacted with both this protein and cyclin T, indicating that this protein may be involved in AIDS. It is also known that CDK9 participates in the differentiation of skeletal muscle and forms associations with other proteins. The customized CDK9 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The CDK9 KO cell line enable us to develop new insights into its role in disease pathology. Apr 29 2022

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We have found this CDK9 KO cell line to be highly reliable and robust, producing consistent results. Mar 10 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CDK9 expression in chordoma cell lines and tissues.

Expression of CDK9 in cell lines of chordoma. Expression of CDK9 in tissues of chordomas. The CDK9 protein exists in two isoforms: the 42 KD isoform and the 55 KD isoform. The first isoform to be discovered was the smaller 42 KD isoform. CDK9 (55KD band) expression was adjusted to α-Tubulin.

Ref: Shen, Shen, et al. "Aberrant CDK9 expression within chordoma tissues and the therapeutic potential of a selective CDK9 inhibitor LDC000067." Journal of Cancer 11.1 (2020): 132.

Pubmed: 31892980

DOI: 10.7150/jca.35426

Research Highlights

With the aim of enhancing efficacy and safety, predictive biomarkers that may assist in identifying patients most likely to react to CDK9 inhibitors are now being used, given that CDK9 inhibitors are nonselective.
Boffo, Silvia, et al. "CDK9 inhibitors in acute myeloid leukemia." Journal of Experimental & Clinical Cancer Research 37 (2018): 1-10.
Pubmed: 29471852 DOI: 10.1186/s13046-018-0704-8

This study offers a thorough analysis of CDK9's biology and structure, as well as an updated list of the inhibitors that are currently being studied in preclinical and clinical settings. It also discusses the significance of CDK9 in solid and hematological tumors.
Anshabo, Abel Tesfaye, et al. "CDK9: a comprehensive review of its biology, and its role as a potential target for anti-cancer agents." Frontiers in oncology 11 (2021): 678559.
Pubmed: 34041038 DOI: 10.3389/fonc.2021.678559