mProX™ Human CDK8 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1076	Magic™ Human CDK8/CycC in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDK8

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Intellectual Developmental Disorder With Hypotonia And Behavioral Abnormalities and Ebstein Anomaly. Among its related pathways are Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants and Gene expression (Transcription)

Gene ID

1024

UniProt ID

P49336

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human CDK8 gene encodes the enzyme cell division protein kinase 8. This gene codes for a protein that belongs to the family of cyclin-dependent protein kinases, or CDKs. Through many processes, CDK8 and cyclin C regulate transcription by forming an association with the mediator complex. Many transcription factors are bound by CDK8 and/or phosphorylated, which can either activate or inhibit the action of the transcription factors. SREBP, STAT1 S727, and the Notch intracellular domain are all phosphorylated by CDK8. Additionally, through affecting the turnover of subunits in the mediator complex tail module, CDK8 prevents transcriptional activation. Furthermore, RNA polymerase II's interaction to the mediator complex is influenced by CDK8. The customized CDK8 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Sharon

This human CDK8 overexpression cell line is sensitive and specific. I definitely recommend this cell line. Feb 19 2020

chat Verified Customer

chat Deborah

I must say that I am extremely impressed with its performance. The CDK8 cell line was easy to use and provided excellent results in my experiments. Jun 14 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Western blot analysis of colon cancer cell lines for CDK8 and β-catenin.

Every cell line exhibited strong expressions of β-catenin and CDK8. Each cell line showed an increase in β-catenin levels but a decrease in CDK8 levels following a 24-hour serum deprivation.

Ref: Seo, Jong-Og, Song Iy Han, and Sung-Chul Lim. "Role of CDK8 and β-catenin in colorectal adenocarcinoma." Oncology reports 24.1 (2010): 285-291.

Pubmed: 20514474

Research Highlights

These findings imply that, in line with CDK8's function in chemotherapy-induced tumor-supporting paracrine activities, the expression of CDK8 and its interacting genes has a significant influence on the response to adjuvant therapy against breast cancer.
V Broude, Eugenia, et al. "Expression of CDK8 and CDK8-interacting genes as potential biomarkers in breast cancer." Current cancer drug targets 15.8 (2015): 739-749.
Pubmed: 26452386 DOI: 10.2174/156800961508151001105814

By joining the mediator complex or phosphorylating transcription factors (TF), cyclin-dependent kinases 8 (CDK8) controls the transcriptional process. Numerous malignancies have been reported to overexpress CDK8.
Xi, Meiyang, et al. "CDK8 as a therapeutic target for cancers and recent developments in discovery of CDK8 inhibitors." European Journal of Medicinal Chemistry 164 (2019): 77-91.
Pubmed: 30594029 DOI: 10.1016/j.ejmech.2018.11.076