mProX™ Human CDK7 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1075	Magic™ Human CDK7/CycH/MAT1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDK7

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Pigmentation Research

Related Diseases

Trichothiodystrophy and Xeroderma Pigmentosum, Variant Type. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and RNA Polymerase II Transcription Initiation And Promoter Clearance

Gene ID

1022

UniProt ID

P50613

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Humans have an enzyme called cyclin-dependent kinase 7, or cell division protein kinase 7. The CDK7 gene encodes this enzyme. This gene codes for a protein that belongs to the family of cyclin-dependent protein kinases, or CDKs. Members of the CDK family, who are known to be significant regulators of cell cycle progression, are very similar to the gene products of Saccharomyces cerevisiae cdc28 and Schizosaccharomyces pombe cdc2. Together with cyclin H and MAT1, this protein forms a trimeric complex that activates Cdk (CAK). It is a crucial part of the transcription factor TFIIH, which is involved in DNA repair and transcription initiation. It is believed that this protein acts as a direct conduit between the cell cycle and transcription control. The customized CDK7 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Patricia

Using the mouse CDK7 cell line from Creative Biolabs has been a game-changer for our research. Feb 09 2021

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chat Brian

It was instrumental in our study, allowing us to specifically target CDK7 proteins and investigate their role in disease. Dec 11 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Effects of CDK7 inhibitors on cells with acquired resistance to WZ4002 or osimertinib.

Phosphorylation of RNAPII's CTD contributes to transcription initiation and RNAPII procession, and CDK7 kinase activity is implicated in this process. Similar effects of THZ1 were seen in H1975 and H1975/OR cells on RNAPII-CTD activity. At the lowest concentration of THZ1, the H1975/WR cells, however, showed suppression of RNAPII-CTD phosphorylation at Ser2, Ser5, and Ser7. Furthermore, EGFR or Akt activity was not inhibited by THZ1 therapy, whereas Erk activity exhibited a dose-dependent increase.

Ref: Ji, Wonjun, et al. "Efficacy of the CDK7 inhibitor on EMT-associated resistance to 3rd generation EGFR-TKIs in non-small cell lung cancer cell lines." Cells 9.12 (2020): 2596.

Pubmed: 33287368

DOI: 10.3390/cells9122596

Research Highlights

Cyclin H, RING-finger protein MAT1, and cyclin-dependent kinase 7 (CDK7) combine to create the heterotrimeric CDK-activating kinase (CAK) complex, which is essential for transcription and cell-cycle regulation.
Peissert, Stefan, et al. "Structural basis for CDK7 activation by MAT1 and Cyclin H." Proceedings of the National Academy of Sciences 117.43 (2020): 26739-26748.
Pubmed: 33055219 DOI: 10.1073/pnas.2010885117

In addition to being an effector CDK that phosphorylates Pol II and other targets within the transcriptional machinery, Cdk7 is also a CDK-activating kinase (CAK) for at least one other crucial CDK implicated in transcription. Cdk7 is linked to the transcription initiation factor TFIIH.
Fisher, Robert P. "Cdk7: a kinase at the core of transcription and in the crosshairs of cancer drug discovery." Transcription 10.2 (2019): 47-56.
Pubmed: 30488763 DOI: 10.1080/21541264.2018.1553483