mProX™ Human CDK5 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1071	Magic™ Human CDK5/p25NCK in Vitro Assay	Human		Kinase Assay
S01YF-1122-KX1072	Magic™ Human CDK5/p35NCK in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDK5

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HGC-27; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Lissencephaly 7 With Cerebellar Hypoplasia and Lissencephaly. Among its related pathways are Gene expression (Transcription) and Defective Intrinsic Pathway for Apoptosis

Gene ID

1020

UniProt ID

Q00535

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The Cdk5 gene encodes cyclin-dependent kinase 5, which is a protein-more precisely, an enzyme. The molecule is a member of the family of cyclin-dependent kinases. Enzymes called kinases catalyze phosphorylation processes. Through this action, an organic substance known as ATP donates a phosphate group to the substrate. Because phosphorylations play a critical role in glycolysis, kinases are an integral component of the cell because they are involved in metabolism, cell signaling, and numerous other functions. The aberrant phosphorylation of tau is a pathogenic action carried out by this kinase, and the consequences are neurofibrillary tangles. The Cdk5 is the chemical explanation for a wide range of neurological illnesses. The customized CDK5 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Kevin

A very stable CDK5 overexpression cell line, especially as there has not been much variation change during my use. Mar 06 2023

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chat Charles

If you are looking for a good value CDK5 cell line for drug discovery, this is the one. Jan 02 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Function of CDK5 in different subcellular locations.

The current study first overexpressed EGFP-CDK5-WT, EGFP-CDK5-NLS, EGFP-CDK5-NES, or p-TRE-EGFP in the HGC-27 GC cell line in order to investigate the function of CDK5 in various subcellular sites. Then, CCK-8 and SRB experiments were run, and the results showed that nuclear CDK5 expression dramatically reduced cell proliferation and colony formation.

Ref: Lu, Jun, et al. "CDK5 suppresses the metastasis of gastric cancer cells by interacting with and regulating PP2A." Oncology Reports 41.2 (2019): 779-788.

Pubmed: 30431123

DOI: 10.3892/or.2018.6860

Research Highlights

Recent discoveries on the molecular interactions that control CDK5 activity and the pathways connected to CDK5 that are linked to a number of disorders are included in this review.
Shupp, Alison, Mathew C. Casimiro, and Richard G. Pestell. "Biological functions of CDK5 and potential CDK5 targeted clinical treatments." Oncotarget 8.10 (2017): 17373.
Pubmed: 28077789 DOI: 10.18632/oncotarget.14538

When cells are treated with the CDK5 inhibitor roscovitine, they become more susceptible to heat-induced apoptosis and its phosphorylation, which stops the actions of apoptotic proteins.
Roufayel, Rabih, and Nimer Murshid. "CDK5: key regulator of apoptosis and cell survival." Biomedicines 7.4 (2019): 88.
Pubmed: 31698798 DOI: 10.3390/biomedicines7040088