mProX™ Human CDK12 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1060	Magic™ Human CDK12wt/CycK in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CDK12

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HCT116; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research; Cancer Research; Reproductive Research

Related Diseases

Lung Cancer Susceptibility 3 and Congenital Heart Defects, Dysmorphic Facial Features, And Intellectual Developmental Disorder. Among its related pathways are Gene expression (Transcription) and Formation of HIV elongation complex in the absence of HIV Tat.

Gene ID

51755

UniProt ID

Q9NYV4

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Cyclin Dependent Kinase 12 Protein is encoded by CDK12. Lung cancer susceptibility, congenital heart defects, dysmorphic facial features, and intellectual developmental disorder are diseases linked to CDK12. Gene expression (transcription) and the formation of the HIV elongation complex in the absence of HIV Tat are two of its associated mechanisms. Cyclin-dependent kinase that plays a crucial role in controlling transcription elongation by phosphorylating the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A). controls the expression of genes necessary for DNA repair and is necessary to keep the genome stable. necessary for RNA splicing; phosphorylation of SRSF1/SF2 may be the mechanism. involved in controlling the activity of MAP kinase, which may impact how the body reacts to estrogen inhibitors. The customized CDK12 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jason

The human CDK12 cell line was an indispensable tool in my research. The cell line's stability and reproducibility were outstanding, allowing for reliable results across multiple experiments. Mar 18 2023

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chat Cynthia

The CDK12 cell line is of high quality and performs exceptionally well in my experiments. Dec 29 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CDK12/13 inhibition results in selective cytotoxicity in NB cells and affects transcription elongation.

Human NB cells treated with THZ531 at escalating doses for 72 hours showed dose-response curves. Included as well were Kelly E9R cells, which exhibit a homozygous mutation at the Cys1039 THZ531-binding region in CDK1220.

Ref: Krajewska, Malgorzata, et al. "CDK12 loss in cancer cells affects DNA damage response genes through premature cleavage and polyadenylation." Nature communications 10.1 (2019): 1757.

Pubmed: 30988284

DOI: 10.1038/s41467-019-09703-y

Research Highlights

As a member of the cyclin-dependent kinase (CDK) family of serine/threonine protein kinases, cyclin-dependent kinase 12 (CDK12) controls transcriptional and post-transcriptional processes, which in turn affects a variety of cellular functions.
Lui, Goldie YL, Carla Grandori, and Christopher J. Kemp. "CDK12: an emerging therapeutic target for cancer." Journal of clinical pathology 71.11 (2018): 957-962.
Pubmed: 30104286 DOI: 10.1136/jclinpath-2018-205356

A growing therapeutic target, cyclin-dependent kinase 12 (CDK12) controls the transcription of DNA-damage response (DDR) genes.
Jiang, Baishan, et al. "Discovery and resistance mechanism of a selective CDK12 degrader." Nature chemical biology 17.6 (2021): 675-683.
Pubmed: 33753926 DOI: 10.1038/s41589-021-00765-y