mProX™ Human CD14 Stable Cell Line

Product Information

Target Family

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;MGC-803

Target Classification

CD Cell Lines

Target Research Area

Orphan Receptor Research

Related Diseases

Mycobacterium Chelonae; Croup

Gene ID

Human:929

UniProt ID

Human:P08571

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

CD14 is a protein that plays a role in various biological processes and has applications in different fields of research. In the context of synovial fluid in arthritis, CD14 dim , OPG + , ZO-1 + cells consistent with resident synovial macrophages (RSM) were identified. These RSM are migratory and can initiate or coordinate both acute (septic) or chronic (autoimmune or inflammatory) arthritis. In neuromyelitis optica spectrum disorder (NMOSD), excessive expression of MALAT1, an upstream target of miR-30b-5p, leads to upregulation of BAFF expression. This promotes the immunologic process of NMOSD by stimulating B cell activation and proliferation. CD14+ monocytes in peripheral blood mononuclear cells (PBMCs) mediate the anti-inflammatory effects of n-3 PUFA-rich lipid emulsions in parenteral nutrition. These lipid emulsions increase the expression of CD1d and CD86 in CD14+ monocytes, leading to increased IL-10 production and reduced pro-inflammatory cytokines. In long-COVID, CD14+ monocytes and NKT cells show altered expression and interaction, contributing to long-term pulmonary dysfunction and metabolic syndrome. In metabolic dysfunction-associated fatty liver disease (MAFLD), CD14+ monocytes differentiate into monocyte-derived macrophages (MdMs) that accumulate in the liver. These MdMs participate in lipid uptake during early stages of MAFLD. Overall, CD14 has implications in immunoregulation, autoimmune disorders, nutrition, pulmonary function, and liver disease.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Cameron Davis (Verified Customer)

How does CD14 function in immune responses? Mar 05 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

CD14 plays a critical role in immune responses, particularly in recognizing and binding lipopolysaccharides, and is involved in the pathogenesis of inflammation and various diseases. Mar 05 2022

chat Alex Davis (Verified Customer)

Can CD14 expression be influenced by external factors? Apr 26 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

External factors like honey bee venom and certain drugs can modulate CD14 expression, impacting monocyte differentiation and immune responses. Apr 26 2021

Published Data

Fig.1 Knockdown of CD14 in gastric cancer cell lines.

A stable reduction in CD14 expression was achieved in gastric cancer MGC-803 cells, with detection conducted through RT-PCR and western blotting. Significantly lower levels (P<0.01) were observed when compared to cells transfected with negative control shRNA, and this was noted in three independent experiments. CD14, identified as cluster of differentiation 14, was the target of the knockdown.

Ref: Li, Kang, et al. "CD14 regulates gastric cancer cell epithelial‑mesenchymal transition and invasion in vitro." Oncology reports 30.6 (2013): 2725-2732.

Pubmed: 24064911

DOI: 10.3892/or.2013.2733

Research Highlights

Sanhueza, Sergio. et al. "Clinical and pulmonary function analysis in long-COVID revealed that long-term pulmonary dysfunction is associated with vascular inflammation pathways and metabolic syndrome." Frontiers in medicine, 2023.
Long-term pulmonary dysfunction (L-TPD), a crucial symptom of long-COVID, has been linked to disease severity and prior comorbidities during the acute phase. However, there is currently limited understanding of the specific clinical manifestations, associated consequences, and underlying molecular pathways of this condition. The objective of this research was to investigate and describe L-TPD in individuals with long-COVID, as well as identify the predominant pathways and long-term effects of this condition through comprehensive analysis of clinical parameters and functional assessments, aided by machine learning and serum proteome profiling.
Sanhueza, Sergio. et al. "Clinical and pulmonary function analysis in long-COVID revealed that long-term pulmonary dysfunction is associated with vascular inflammation pathways and metabolic syndrome." Frontiers in medicine, 2023.
Pubmed: 37869162 DOI: 10.3389/fmed.2023.1271863

M Chan, Mandy. et al. "Steatosis drives monocyte-derived macrophage accumulation in human metabolic dysfunction-associated fatty liver disease." JHEP reports : innovation in hepatology, 2023.
The study of metabolic dysfunction-associated fatty liver disease (MAFLD) has recently focused on the role of macrophage composition in hepatic steatosis, a common complication of obesity. Animal models have revealed significant changes in macrophage heterogeneity in MAFLD, but the connection between liver macrophages and steatosis remains inconclusive in human subjects. Thus, further research is needed to fully understand the relationship between liver macrophages and fatty liver disease in humans.
M Chan, Mandy. et al. "Steatosis drives monocyte-derived macrophage accumulation in human metabolic dysfunction-associated fatty liver disease." JHEP reports : innovation in hepatology, 2023.
Pubmed: 37869071 DOI: 10.1016/j.jhepr.2023.100877