mProX™ Human CAMKK2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1055	Magic™ Human CAMKK2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CAMKK2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research

Related Diseases

Parkinson Disease, Late-Onset. Among its related pathways are Calmodulin induced events and GPCR downstream signalling

Gene ID

10645

UniProt ID

Q96RR4

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The enzyme calcium/calmodulin-dependent protein kinase kinase 2 is encoded by the CAMKK2 gene in humans. The gene's product is a member of the Ca++/calmodulin-dependent protein kinase subfamily and the serine/threonine-specific protein kinase family. By phosphorylating the downstream kinases CaMK1 and CaMK4, this protein contributes to the calcium/calmodulin-dependent (CaM) kinase cascade by enhancing their catalytic activity. CaMKK2 is an AMPK kinase in the hypothalamus that controls the synthesis of the hormone neuropeptide Y, which stimulates appetite. Through the downregulation of mu opioid receptors and the decrease of downstream signaling pathways, it also plays a significant role in the development of hyperalgesia and tolerance to opioid analgesic medications. The customized CAMKK2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Thomas

The CAMKK2 overexpression cell line design is well-optimized, and the provided documentation is comprehensive. It has saved me time and effort compared to traditional methods. Mar 09 2023

chat Verified Customer

chat Karen

Most importantly, I would recommend this product to anyone who wants to research CAMKK2. Oct 16 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CaMKK2 is expressed in mammary tumor-associated stromal cells

Although there was a strong positive association found between the expression of CaMKK2 in tumors and stromal cells, overexpression of the protein did not correlate with the grade of the tumor. CaMKK2 may therefore have both intrinsic and extrinsic effects on tumor pathobiology in cancer cells.

Ref: Racioppi, Luigi, et al. "CaMKK2 in myeloid cells is a key regulator of the immune-suppressive microenvironment in breast cancer." Nature Communications 10.1 (2019): 2450.

Pubmed: 31164648

DOI: 10.1038/s41467-019-10424-5

Research Highlights

Understanding the ways in which Ca(2+)/CaM-dependent signaling affects metabolic balance and illness requires a grasp of this information.
Marcelo, Kathrina L., Anthony R. Means, and Brian York. "The Ca2+/calmodulin/CaMKK2 axis: nature's metabolic CaMshaft." Trends in Endocrinology & Metabolism 27.10 (2016): 706-718.
Pubmed: 27449752 DOI: 10.1016/j.tem.2016.06.001

Skeletal health declines as a result of oxidative stress, inflammation, and the buildup of advanced glycation endproducts (AGEs) brought on by age and metabolic diseases. In these circumstances, bone loses some of its ability to regenerate and becomes more prone to fractures.
Williams, Justin N., and Uma Sankar. "CaMKK2 signaling in metabolism and skeletal disease: a new axis with therapeutic potential." Current osteoporosis reports 17 (2019): 169-177.
Pubmed: 31115859 DOI: 10.1007/s11914-019-00518-w