mProX™ Human CAMK2A Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1050	Magic™ Human CaMK2α(CAMK2A) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

CAMK2A

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

A549; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Intellectual Developmental Disorder, Autosomal Recessive 63 and Intellectual Developmental Disorder, Autosomal Dominant 53. Among its related pathways are Calmodulin induced events and Signaling downstream of RAS mutants

Gene ID

815

UniProt ID

Q9UQM7

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The enzyme that the CAMK2A gene produces is a member of the Ca2+/calmodulin-dependent protein kinase II subfamily and the serine/threonine-specific protein kinase family. At glutamatergic synapses, Ca2+ signaling is essential for multiple aspects of synaptic plasticity. The four chains that make up this enzyme are delta, gamma, beta, and alpha. Both spatial learning and hippocampus long-term potentiation (LTP) depend on the alpha chain expressed by this gene. This protein can be autophosphorylated to produce CaM-independent activity in addition to its calcium-calmodulin (CaM)-dependent activity. For this gene, two transcript variants encoding different isoforms have been found. The customized CAMK2A stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Carol

The CAMK2A cell line was delivered promptly, and it performed exceptionally well in my assays. Jan 03 2020

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chat David

The mouse CAMK2A KO cell line is easy to handle and has shown consistent performance in various experimental setups. Dec 13 2020

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FAQ

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Published Data

Fig.1 CAMK2A enhanced SOX2 expression through decreasing EZH2 mediated H3K27me3 in the regulatory region of SOX2.

The restrictive histone mark H3K27me3 was considerably elevated by CAMK2A-KD in both HCC827 and PDCL#24 when compared to controls; however, there was no significant change in the repressive histone mark H3K27me3 trimethylation on EZH2. In contrast, H3K27me3 was significantly repressed whereas EZH2 was only marginally decreased by CAMK2A-OE.

Ref: Wang, Si-Qi, et al. "CAMK2A supported tumor initiating cells of lung adenocarcinoma by upregulating SOX2 through EZH2 phosphorylation." Cell Death & Disease 11.6 (2020): 410.

Pubmed: 32483123

DOI: 10.1038/s41419-020-2553-6

Research Highlights

This de novo CAMK2A mutation associated with ASD impairs several CaMKII activities, results in synaptic impairments, and alters behavior associated with ASD. These findings offer new insights into the synaptic mechanisms underlying ASD.
Stephenson, Jason R., et al. "A novel human CAMK2A mutation disrupts dendritic morphology and synaptic transmission, and causes ASD-related behaviors." Journal of Neuroscience 37.8 (2017): 2216-2233.
Pubmed: 28130356 DOI: 10.1523/JNEUROSCI.2068-16.2017

These findings imply that malfunctioning CAMK2 paralogs may be involved in various neurological illnesses and show that a recessive germline mutation in CAMK2A causes neurodevelopmental abnormalities in people.
Chia, Poh Hui, et al. "A homozygous loss-of-function CAMK2A mutation causes growth delay, frequent seizures and severe intellectual disability." Elife 7 (2018): e32451.
Pubmed: 29784083 DOI: 10.7554/eLife.32451