mProX™ Human CALCRL Stable Cell Line

Product Information

Target Protein

CALCRL

Target Family

Calcitonin Family

Target Protein Species

Human

Host Cell Type

MOLM-14;OCI-AML2;OCI-AML3;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Immunology Research

Related Diseases

Lymphatic Malformation 8;Hydrops Fetalis, Nonimmune

Gene ID

Human: 10203

UniProt ID

Human: Q16602

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The Calcitonin Receptor-Like Receptor (CALCRL) has garnered attention in the scientific domain due to its association with various physiological processes. One of the notable findings is its involvement with the adrenomedullin signaling pathway, which has implications in acute myeloid leukemia (AML). Targeting the adrenomedullin-CALCRL axis has been suggested to improve chemotherapy responses in AML models, underscoring its potential as a therapeutic target. Additionally, research has indicated that CALCRL can predict outcomes in AML beyond existing risk factors. Another intriguing aspect of CALCRL research is its role in the lung microenvironment, especially in the context of shock and acute lung injury. The exploration of CALCRL in scientific research has provided valuable insights into its multifunctional roles, from cancer biology to respiratory physiology. Its diverse functions make it a subject of ongoing investigations, with the potential to unveil novel therapeutic strategies for various ailments.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Gary (Verified Customer)

How does CALCRL contribute to cell proliferation and migration? Oct 21 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

CALCRL can activate specific signaling cascades, leading to cell proliferation, migration, and adhesion, especially in the context of trophoblast cells. Oct 21 2020

chat Patricia (Verified Customer)

Are there any known receptors or signaling pathways associated with CALCRL? Sep 10 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

Yes, the ADM receptor component CALCRL is known to activate the AKT-TSC2-MTOR, particularly the MTORC1 signaling cascade. Sep 10 2021

Published Data

Fig.1 The CALCRL-ADM axis is required for cell growth and survival

MOLM-14, OCI-AML2, and OCI-AML3 cell lines were transfected with either control shRNA or shCALCRL. The graph illustrates the proportion of Annexin-V+ or 7-AAD+ cells four days post-transduction (n = 3 independent trials for MOLM-14 and OCI-AML3, and n = 4 independent trials for OCI-AML2; unpaired t-test analysis).

Ref: Larrue, Clément, et al. "Adrenomedullin-CALCRL axis controls relapse-initiating drug tolerant acute myeloid leukemia cells." Nature Communications 12.1 (2021): 422.

Pubmed: 33462236

DOI: 10.1038/s41467-020-20717-9

Research Highlights

Surakka I, et al. "Multi-ancestry meta-analysis identifies 5 novel loci for ischemic stroke and ." Cell genomics, 2023.
Stroke is globally one of the leading causes of death and disability. Its incidence and risk differ based on ethnicity and gender, which may be attributed to genetic variations among different subgroups. Through a collaboration involving 16 biobanks across various ancestries, the genetics of ischemic stroke (60,176 cases and 1,310,725 controls) were studied under the Global Biobank Meta-analysis Initiative (GBMI) and in conjunction with previously published MegaStroke data. Further analysis revealed 5 new genetic loci associated with ischemic stroke (LAMC1, CALCRL, PLSCR1, CDKN1A, and SWAP70) after validation in 4 more datasets. The ABO locus previously reported displayed significant diversity among ancestries, while the ALDH2 locus showed significant sex variance (males p = 1.67e-24, females p = 0.126) and was observed solely in East Asian male subjects. These findings highlight the importance of larger and more diverse datasets for a better understanding of the genetic factors influencing stroke risk in various gender and ancestry groups.
Pubmed: 37601974 DOI: 10.1016/j.xgen.2023.100345

Tu NH, et al. "Calcitonin Related Polypeptide Alpha Mediates Oral Cancer Pain.." Cells, 2023.
The study investigated the role of Calcitonin gene related polypeptide (CGRP) in oral cancer pain. Through the use of single-cell RNA-sequencing, the researchers identified potential cell types expressing the CGRP receptor components, Ramp1 and Calcrl, in the cancer microenvironment. CGRP was found to be present in the nerves and axons innervating oral cancer tumors in mice and was shown to contribute to neurogenic secretion in the oral cancer microenvironment. In preclinical models, CGRP antagonism was effective in reversing oral cancer pain. These findings suggest that targeting CGRP may provide relief for oral cancer patients suffering from pain.
Pubmed: 37443709 DOI: 10.3390/cells12131675