mProX™ Human ADRA2C Stable Cell Line

Zhou H, et al. "Chronic unpredictable stress induces depression/anxiety-related behaviors and ." Heliyon, 2023.
Depression and anxiety are two of the most prevalent mental health disorders, affecting individuals of all ages but with a higher incidence among females. However, there is limited understanding of the underlying physiological changes associated with these conditions. In this study, the impact of age and stress on depression- and anxiety-related behaviors in female mice was investigated. Using the saccharin preference and open field test, the authors measured behavioral changes in 4-, 14- and 25-month-old mice before and after exposure to chronic unpredictable stress. Additionally, gene expression levels of monoamine receptors in the hippocampus were analyzed using real-time RT-PCR. Results showed that chronic unpredictable stress decreased saccharin preference in all age groups and time spent in the center in the open field test in 25-month-old mice. Analysis of variance revealed significant age and stress effects on mRNA levels of several monoamine receptors, as well as interactions between age and stress. Further analysis showed that chronic unpredictable stress decreased mRNA levels of specific receptors in 4-month-old mice. Correlations were also found between saccharin preference and mRNA levels of certain receptors in 4-month-old mice, and between time spent in the center in the open field test and mRNA levels of other receptors in 4- and 14-month-old mice. Overall, these findings suggest that stress can trigger depression- and anxiety-related behaviors and influence the expression of hippocampal monoamine receptors in a manner that is dependent on the age of female mice.
Pubmed: 37539192   DOI: 10.1016/j.heliyon.2023.e18369

Woodman R, et al. "Alpha-methyltyrosine reduces the acute cardiovascular and behavioral sequelae in ." The journal of trauma and acute care surgery, 2023.
In the study conducted, it was found that increased catecholamines after traumatic brain injury (TBI) can lead to adverse outcomes such as heightened cardiovascular reactivity and behavioral deficits. The use of adrenergic receptor blockers to reduce these negative effects has not been very successful. The inhibition of catecholamine synthesis using alpha-methyltyrosine (alphaMT) showed potential benefits for TBI. The study, which involved randomized trials on mice, evaluated the effects of alphaMT (50 mg.kg -1 .d -1 ) versus a control group after TBI induced by controlled cortical impact. Results showed that alphaMT was able to block TBI-induced increases in blood pressure and cardiac reactivity. It also decreased blood brain barrier leakage and improved behavioral outcomes. Furthermore, alphaMT prevented the induction of the Nkcc1 gene following TBI and diminished Ae3 gene transcription. These findings suggest that alphaMT may be effective in treating TBI by regulating gene expressions and minimizing catecholamine and sympathetic output. The study also revealed a new relationship between Kcc2 and the chloride/bicarbonate exchanger, which could be important in future trials targeting central intraneuronal chloride concentrations after acute brain injury.
Pubmed: 37165479   DOI: 10.1097/TA.0000000000004023