mProX™ Human ADGRL4 Stable Cell Line

Product Information

Target Protein

ADGRL4

Target Family

Orphan Family

Target Protein Species

Human

Host Cell Type

SMMC-7721; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Ocular Research; Orphan Receptor Research

Related Diseases

Transient Refractive Change; Volkmann Contracture

Gene ID

64123

UniProt ID

Q9HBW9

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Smooth muscle cells and endothelial cells both express the orphan adhesion GPCR ADGRL4/ELTD1, which regulates both healthy and malignant angiogenesis. Zebrafish embryos with vascular abnormalities that were unable to support life were created when in vitro endothelium sprouting was blocked. ADGRL4/ELTD1 is overexpressed compared to the surrounding normal endothelium in tumor-associated endothelial cells from a variety of common tumor types (head and neck, renal, colorectal, ovarian, and brain malignancies), as well as from glioblastoma tumor cells. Systemic vascular ADGRL4/ELTD1 silencing lowered microvascular density and generated tumor shrinkage with improved survival in mice implanted with human tumor xenografts (colorectal and ovarian tumors). This was done without having a deleterious impact on the systemic vascular system. One explanation is that ADGRL4/ELTD1 may increase tumor drug administration by stabilizing blood vessels. Due to this, ADGRL4/ELTD1 is a important drug target. The customized ADGRL4 stable cell line can be employed in the signaling pathway researches, as well as the screening of potential therapeutic candidates.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Xiaoling (Verified Customer)

Can I request a specific host cell for the ADGRL4 stable cell line? Jun 08 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

Yes, you can select suitable host cells for customized cell line. Jun 08 2023

chat Bruce (Verified Customer)

What is the QCs for this cell line? Jun 16 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

The QCs include testing for bacterial and mycoplasma contamination, flow cytometry to verify the surface expression, and qPCR to confirm the engineering results. Jun 16 2023

Published Data

Fig.1 ELTD1 expression in a normal liver cell line and HCC cell lines.

(A) Western blotting and qPCR data reveal that Huh7 has significant ELTD1 expression relative to the typical liver cell line L02, while SMMC-7721 cells exhibit low expression. (B) Western blotting and qPCR results showing that the SMMC-7721 cell line overexpresses ELTD1. (C) Huh7 cell line with ELTD1 knockdown validated by western blotting and qPCR.

Ref: Kan A., et al. ELTD1 Function in Hepatocellular Carcinoma is Carcinoma-Associated Fibroblast-Dependent. J Cancer. 2018, 9(14):2415-2427.

Pubmed: 30026838

DOI: 10.7150/jca.24406

Research Highlights

An orphan adhesion GPCR (aGPCR) called ADGRL4/ELTD1 controls tumor angiogenesis in endothelial cells.
Favara, D. M., et al. Elevated expression of the adhesion GPCR ADGRL4/ELTD1 promotes endothelial sprouting angiogenesis without activating canonical GPCR signalling. Scientific reports. 2021, 11(1), 1-13.
Pubmed: 33893326 DOI: 10.1038/s41598-021-85408-x

The endothelial cell adhesion G protein-coupled receptor (aGPCR) known as adhesion G protein-coupled receptor L4 (ADGRL4/ELTD1) controls normal and tumor angiogenesis, making it a prime target for anti-cancer treatments.
Favara, D. M., et al. ADGRL4/ELTD1 silencing in endothelial cells induces ACLY and SLC25A1 and alters the cellular metabolic profile. Metabolites. 2019, 9(12), 287.
Pubmed: 31775252 DOI: 10.3390/metabo9120287

A favourable prognosis is associated with higher ELTD1/ADGRL4 expression in the vasculature of several tumor types.
Sheldon, H., et al. ADGRL4/ELTD1 Expression in Breast Cancer Cells Induces Vascular Normalization and Immune SuppressionELTD1 Is Angiogenic and Immunosuppressive in Breast Cancer. Molecular Cancer Research. 2021, 19(11), 1957-1969.
Pubmed: 34348993 DOI: 10.1158/1541-7786.MCR-21-0171