mProX™ Human ADGRB3 Stable Cell Line

Product Information

Target Protein

ADGRB3

Target Family

Orphan Family

Target Protein Species

Human

Host Cell Type

MB; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cardiovascular Research; CNS Research; Orphan Receptor Research

Related Diseases

Carey-Fineman-Ziter Syndrome; Polymicrogyria; Bilateral Frontoparietal

Gene ID

577

UniProt ID

O60242

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

ADGRB3 is a member of the adhesion G protein-coupled receptor (adhesion GPCR) subfamily, which is important in the control of a number of CNS processes, including axon guidance, myelination, and synapse development and function. ADGRB3 is similarly extensively expressed in the cerebellum, and ADGRB1 and ADGRB3 are concentrated in the postsynaptic density. ADGRB3 interacts with C1q3, another secreted protein that is particular to the brain, to regulate synapse density in hippocampal neurons where it is also highly expressed. In fact, mice with hippocampus neurons lacking ADGRB3 exhibit severely impaired dendritic formation. It's interesting to note that ADGRB3 has already been linked to drug addiction, bipolar disorder, and schizophrenia. The customized ADGRB3 stable cell line can be employed in the discovery and development of antibodies, signaling pathway researches, as well as the screening of potential therapeutic candidates.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat William (Verified Customer)

Can you provide tips to reduce the background noise? Mar 08 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

Use healthy cells that have been passaged at least 24 hours before to the assay.
Don't let the cells reach 80% confluence.
Don't put too many cells in each well. Mar 08 2023

chat John (Verified Customer)

What freezing medium should be used for this cell line? Mar 23 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

The use of frozen culture depends on the host cell type. For adherent cells, we recommend DMEM, 20% (v/v) fetal bovine serum (FBS), and 10% (v/v) DMSO. For suspension cells, we recommend fetal bovine serum (FBS) and 10% DMSO. Mar 23 2023

Published Data

Fig.1 Resolution of heteroduplex bands as a function of migration duration and agarose concentration.

Time course of electrophoresis of PCR products on 4% agarose gels for samples with a 7-bp deletion, a 1-bp insertion, and a 3-bp insertion in the Adgrb3 gene (196-bp amplicon) and Scn8a gene (186-bp amplicon), respectively.

Ref: Bhattacharya, Debanjan, and Erwin G. Van Meir. "A simple genotyping method to detect small CRISPR-Cas9 induced indels by agarose gel electrophoresis." Scientific reports 9.1 (2019): 4437.

Pubmed: 30872606

DOI: 10.1038/s41598-019-39950-4

Research Highlights

Although recent large-sample GWASs found significant genes in the background of depression, their applicability is hampered by the variability of the depressive phenotype and the absence of reliable phenotyping.
Gonda, X., et al. Genetic underpinnings of affective temperaments: a pilot GWAS investigation identifies a new genome-wide significant SNP for anxious temperament in ADGRB3 gene. Translational psychiatry. 2021, 11(1), 1-15.
Pubmed: 34075027 DOI: 10.1038/s41398-021-01436-1

The first diagnostic procedure for undiagnosed neurodevelopmental problems, chromosomal microarray analysis has recently enabled the identification of rearrangements underlying a number of neurodevelopmental illnesses.
Scuderi, C., et al. Biallelic intragenic duplication in ADGRB3 (BAI3) gene associated with intellectual disability, cerebellar atrophy, and behavioral disorder. European Journal of Human Genetics. 2019, 27(4), 594-602.
Pubmed: 30659260 DOI: 10.1038/s41431-018-0321-1

Some of the bigger C1q superfamily's proteins function as synaptic organizers in significant neuronal processes in different parts of the brain.
Sticco, M. J., et al. C1QL3 promotes cell-cell adhesion by mediating complex formation between ADGRB3/BAI3 and neuronal pentraxins. The FASEB Journal. 2021, 35(1), e21194.
Pubmed: 33337553 DOI: 10.1096/fj.202000351RR