mProX™ Human ADGRB1 Stable Cell Line

Product Information

Target Protein

ADGRB1

Target Family

Orphan Family

Target Protein Species

Human

Host Cell Type

MB; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cancer Research; Cardiovascular Research; Infectious Research; Orphan Receptor Research

Related Diseases

Glioblastoma; Carey-Fineman-Ziter Syndrome

Gene ID

575

UniProt ID

O14514

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The adhesion GPCR BAI1/ADGRB1 is crucial for mediating phagocytosis, inhibiting angiogenesis, and preventing the growth of brain tumors. Along with regulating the growth of excitatory synapse and dendritic spines, BAI1 interacts with a number of proteins that are important for autism. According to studies, overexpressing BAI1 causes dendrites to retract whereas underexpressing BAI1 causes dendrites to grow more immature and unstable. Continuous dendritic morphological changes and proper PSD-95 (post-synaptic density 95) function are crucial for learning and memory and are frequently affected in neurological and neurodevelopmental conditions that are characterized by hampered social interaction, communication difficulties, and an increase in repetitive behaviors. The customized ADGRB1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Gary (Verified Customer)

Q: Does this cell line overexpress full-length ADGRB1? Mar 12 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

A: The cell line is a customized cell line. You can choose a suitable host cell to overexpress, KO or KD. Mar 12 2022

chat Shirley (Verified Customer)

Q: What is the QC for Human ADGRB1 Stable Cell Line? Jan 27 2020

chat Sherry Smith (Creative Biolabs Scientific Support)

A: Before shipping, the cell line is examined for bacterial and mycoplasma contamination. The QC also includes flow cytometry to verify the surface expression of ADGRB1 at the protein level and qPCR to verify the engineering results at the mRNA level. Please contact us for specific information. Jan 27 2020

Published Data

Fig.1 BAI1 Inhibits MB Growth In Vitro and In Vivo in a p53-Dependent Manner.

Left: D556 + BAI1 (Tet-on) cell proliferation with stable p53 knockdown following BAI1 induction. Fold is the proportion of living cells on day three to day zero. Right: After p53 shRNA lentivirus had been transfected into ONS-76 MB cells for 5 days, cell proliferation was assessed.

Ref: Zhu, D., et al. BAI1 suppresses medulloblastoma formation by protecting p53 from Mdm2-mediated degradation. Cancer cell. 2018, 33(6), 1004-1016.

Pubmed: 29894688

DOI: 10.1016/j.ccell.2018.05.006

Research Highlights

The subfamily B member BAI1 is widely expressed in the brain, where it suppresses angiogenesis and, according to a recent study, promotes neurogenesis and synaptogenesis.

Hsiao, C. C., et al. Macrophages do not express the phagocytic receptor BAI1/ADGRB1. Frontiers in Immunology. 2019, 10, 962.
Pubmed: 31130954 DOI: 10.3389/fimmu.2019.00962

The adhesion G-protein-coupled receptor B1 (BAI1/ADGRB1) regulates the stability of p53. The ADGRB1 promoter contains histone 3 trimethylated on lysine 27 (H3K27me3), a marker of silent chromatin conformation. By inhibiting EZH2, a catalytic subunit of the Polycomb Repressive Complex 2 (PRC2) that methylates histone 3 on lysine 27, the gene enters an active chromatin status and BAI1 expression is restored.

Zhang, H., et al. EZH2 targeting reduces medulloblastoma growth through epigenetic reactivation of the BAI1/p53 tumor suppressor pathway. Oncogene. 2020, 39(5), 1041-1048.
Pubmed: 31582835 DOI: 10.1038/s41388-019-1036-7