mProX™ Human ACVRL1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1022	Magic™ Human ACVRL1(ALK1) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

ACVRL1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

iPSCs; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research; Reproductive Research

Related Diseases

Telangiectasia, Hereditary Hemorrhagic, Type 2 and Pulmonary Hypertension, Primary, 1. Among its related pathways are ALK1 signaling events and Akt Signaling

Gene ID

UniProt ID

P37023

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The gene ACVRL1 codes for the production of the protein known as activin receptor-like kinase 1. Cell surfaces contain this protein, particularly those lining growing arteries. ACVRL1 is a receptor protein. It functions as a "lock" that waits for a certain protein known as its ligand to act as the "key." The ligand in the instance of the ACVRL1 protein is known as transforming growth factor beta. The formation of blood vessels is influenced by the interactions between these proteins. This protein interaction has a specific role in the development of new blood vessels into veins or arteries. The customized ACVRL1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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We used the ACVRL1 cell line for target validation in our drug development project, and it performed flawlessly. Jan 29 2023

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The ACVRL1 cell line is an essential tool for anyone studying drug discovery. Jan 17 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Generation of embryoid bodies (EBs).

To create embryoid bodies (EBs), ACVRL1wt/wt and ACVRL1wt/mut iPSCs were cultivated in a nonadherent environment. Endothelial differentiation was triggered when EBs got to be about 200 µm in diameter. The diameter of EBs was measured and their growth was tracked.

Ref: Xiang-Tischhauser, Li, et al. "Generation of a Syngeneic Heterozygous ACVRL1 (wt/mut) Knockout iPS Cell Line for the In Vitro Study of HHT2-Associated Angiogenesis." Cells 12.12 (2023): 1600.

Pubmed: 37371070

DOI: 10.3390/cells12121600

Research Highlights

Rendu-Osler-Weber (ROW) syndrome, also known as Hereditary Hemorrhagic Telangiectasia syndrome (HHT), is an autosomal dominant vascular condition. HHT1 and HHT2, the two most prevalent types of HHT, have been associated with mutations in the genes encoding endoglin (ENG) and activin receptor-like kinase 1 (ACVRL1 or ALK1), respectively.
Alaa el Din, Ferdos, et al. "Functional and splicing defect analysis of 23 ACVRL1 mutations in a cohort of patients affected by Hereditary Hemorrhagic Telangiectasia." PLoS One 10.7 (2015): e0132111.
Pubmed: 26176610 DOI: 10.1371/journal.pone.0132111

These findings imply that ALK1 expression in blood vessels is likewise susceptible to the AD pathophysiologic process, possibly connected to CAA, as previously shown for particular neuronal populations. However, in early and moderate AD, cerebral arterioles could still respond to ALK1 ligands like BMP9 and BMP10.
Anderson, Kelley E., et al. "The expression of Activin Receptor-Like Kinase 1 (ACVRL1/ALK1) in hippocampal arterioles declines during progression of Alzheimer's disease." Cerebral Cortex Communications 1.1 (2020): tgaa031.
Pubmed: 32974611 DOI: 10.1093/texcom/tgaa031