mProX™ Human ABL1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Assay Types
S01YF-1122-KX1017	Magic™ Human ABL(ABL1) in Vitro Assay	Human	Kinase Assay
S01YF-1122-KX1018	Magic™ Human ABL1[F317I] in Vitro Assay	Human	Kinase Assay
S01YF-1122-KX1019	Magic™ Human ABL1[T315I] in Vitro Assay	Human	Kinase Assay

Product Information

Target Protein

ABL1

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

K562; Jurkat; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research; Reproductive Research

Related Diseases

Congenital Heart Defects And Skeletal Malformations Syndrome and Leukemia, Chronic Myeloid.Among its related pathways are Development Slit-Robo signaling and Cell Cycle, Mitotic.

Gene ID

UniProt ID

P00519

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The ABL1 gene codes for a protein that is produced in cells all over the body and is involved in numerous functions. The protein ABL1 has kinase activity. In order for the ABL1 kinase to carry out its tasks, it must be turned on from its generally off state. Numerous stimuli can activate the ABL1 kinase, which can phosphate-group add a variety of proteins. Because of its diversity, ABL1 can participate in a large number of different cellular functions. Depending on the state of the cell, it can either promote controlled cell death or help cells survive. The actin cytoskeleton, a web of fibers that forms the internal framework of cells, is mediated by the ABL1 kinase through interactions with several proteins. ABL1 can also aid in controlling the activity of other genes. The customized ABL1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Charlie

The ABL1 cell line is of high quality and performs exceptionally well in my experiments. Dec 28 2022

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chat Vivian

I have purchased this ABL1 cell line and all producing reliable results. Mar 22 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 WASP is downregulated in CML patients and BCR-ABL1-positive cell lines.

Western blot demonstrating WASP expression in cell lines originating from several leukemia subtypes. High amounts of WASP are expressed by BCR-ABL1-negative cell lines, whereas discrete or undetectable WASP is expressed by BCR-ABL1-positive cells. Actin served as a loading regulator.

Ref: Pereira, Welbert O., et al. "BCR-ABL1-induced downregulation of WASP in chronic myeloid leukemia involves epigenetic modification and contributes to malignancy." Cell death & disease 8.10 (2017): e3114-e3114.

Pubmed: 29022901

DOI: 10.1038/cddis.2017.458

Research Highlights

At least one normal ABL1 allele is typically present in leukemias that express constitutively activated mutations of ABL1 tyrosine kinase (BCR-ABL1, TEL-ABL1, NUP214-ABL1).
Dasgupta, Yashodhara, et al. "Normal ABL1 is a tumor suppressor and therapeutic target in human and mouse leukemias expressing oncogenic ABL1 kinases." Blood, The Journal of the American Society of Hematology 127.17 (2016): 2131-2143.
Pubmed: 26864341 DOI: 10.1182/blood-2015-11-681171

The types of tyrosine kinase-activating lesions that have been found were examined in this review, along with the preclinical and clinical data supporting the use of tyrosine kinase inhibitors in the management of this unique subtype of ALL.
Boer, Judith M., and Monique L. den Boer. "BCR-ABL1-like acute lymphoblastic leukaemia: From bench to bedside." European Journal of Cancer 82 (2017): 203-218.
Pubmed: 28709134 DOI: 10.1016/j.ejca.2017.06.012