mProX™ Human ABCG2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-0922-KX1555	Magic™ Human ABCG2 in Vitro Drug screening assay	Human	Caco-2, MDCK, HEK293, CHO, membrane vesicles, and various transfected cells lines	Activity Assay (High throughput screening assays; Large scale single dose, duplicate profiling; IC50 profiling; Ki determination assay; Substrate determination assay )

Product Information

Target Protein

ABCG2

Target Family

ABC Transporter

Target Protein Species

Human

Host Cell Type

NSCLC; CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

Cancer Research; Metabolic Research

Related Diseases

Uric Acid Concentration; Blood Group

Gene ID

9429

UniProt ID

Q9UNQ0

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The ATP-binding cassette gene family, which includes the ABCG2 gene, offers instructions for producing proteins that carry chemicals across cell membranes. The ABCG2 protein aids in the intestines' release (secretion) of urate into the urine. It functions as an antioxidant in the bloodstream, shielding cells from the corrosive effects of unstable molecules known as free radicals. The kidneys and, to a lesser extent, the intestines control the amounts of urea. Certain medications are also transported out of cells via the ABCG2 protein. This protein, for instance, removes certain chemotherapy medications from tissues and organs. These medications' transportation enables both their intended effects and bodily elimination. The customized ABCG2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Ashley

The ABCG2 cell line is a good product for general routine use from Creative Biolabs. Sep 01 2022

chat Verified Customer

chat Gary

I recently purchased the human ABCG2 cell line, and it has been a valuable tool in my laboratory work for drug screening. Jul 06 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Characterization of ABCG2 expression and activity in NSCLC cell lines.

50μg of proteins were put into lysed cells in order to evaluate ABCG2 protein expression using western blot analysis. The information comes from a typical experiment. Three repetitions of each experiment produced findings that were comparable.

Ref: Galetti, Maricla, et al. "Effect of ABCG2/BCRP expression on efflux and uptake of gefitinib in NSCLC cell lines." PloS one 10.11 (2015): e0141795.

Pubmed: 26536031

DOI: 10.1371/journal.pone.0141795

Research Highlights

A constitutively expressed ATP-binding cassette (ABC) transporter, ABCG2 shields a variety of organs from outside substances. Its action impacts the pharmacokinetics of widely used medications and restricts the penetration of treatments into cancerous cells, hence fostering multidrug resistance.
Taylor, Nicholas MI, et al. "Structure of the human multidrug transporter ABCG2." Nature 546.7659 (2017): 504-509.
Pubmed: 28554189 DOI: 10.1038/nature22345

It is acknowledged that ABCG2 plays a significant role in determining the pharmacokinetic properties of the medicines that it substrates. An adequate modification of ABCG2 function during chemotherapy should logically boost the efficacy of anti-cancer medicines by overcoming the MDR phenotype and/or increasing their pharmacokinetics, even though the therapeutic implications of reversing the ABCG2-mediated MDR has proved unconvincing.
Toyoda, Yu, Tappei Takada, and Hiroshi Suzuki. "Inhibitors of human ABCG2: from technical background to recent updates with clinical implications." Frontiers in pharmacology 10 (2019): 208.
Pubmed: 30890942 DOI: 10.3389/fphar.2019.00208