mProX™ Human ABCC3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-0922-KX1558	Magic™ Human ABCC3 in Vitro Drug screening assay	Human	Caco-2, MDCK, HEK293, CHO, membrane vesicles, and various transfected cells lines	Activity Assay (High throughput screening assays; Large scale single dose, duplicate profiling; IC50 profiling; Ki determination assay; Substrate determination assay )

Product Information

Target Protein

ABCC3

Target Family

ABC Transporter

Target Protein Species

Human

Host Cell Type

MCF-7; CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

Digestive and Renal Research; Metabolic Research

Related Diseases

Extrahepatic Cholestasis; Dubin-Johnson Syndrome

Gene ID

8714

UniProt ID

O15438

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

MRP3 (ABCC3) is a transporter protein that facilitates the basolateral export of hepatocyte-produced bile salts and organic anions such as glutathione and glucuronide conjugates. Its affinity and expression in normal liver are low for bile salts. In the livers of people with Dubin-Johnson syndrome and during cholestasis, basolateral MRP3 expression is elevated. Mrp3 is markedly up-regulated in bilirubin-UDP-glucuronosyltransferase-deficient Gunn rats, bile duct-ligated rats, Mrp2-deficient TR− and EHBR rats. The nuclear hormone receptors PXR and CAR that are activated by drugs control MRP3 transcription. Less is known about the natural factors that cause MRP3 to be induced. Therefore, Mrp3 may be triggered by cellular stress caused by lipid peroxidation. The predominant glutathione and glucuronide conjugate overflow system is MRP3. The customized ABCC3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Sandra

This is a very reliable ABCC3 cell line with high sensitivity and specificity, suitable for many experiments. May 19 2021

chat Verified Customer

chat John

Recommended for all labs based on my experience of using this human ABCC3 cell line. May 06 2020

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 ABCC3 is overexpressed in breast cancer samples and cancer cell lines.

The ABCC3 gene expression in breast cancer cell lines (BT-474, T-47D, MCF-7, MDA-MB-231, and HCC-1806) and immortalized breast cell lines (HBL-100 and MCF-10A) assessed by qPCR is displayed in a scatter plot.

Ref: Balaji, Sai A., et al. "Role of the drug transporter ABCC3 in breast cancer chemoresistance." PloS one 11.5 (2016): e0155013.

Pubmed: 27171227

DOI: 10.1371/journal.pone.0155013

Research Highlights

ATP Binding Cassette (ABC) transporters are one of the main mechanisms behind chemoresistance and are often overexpressed in various cancer types.
Adamska, Aleksandra, et al. "ABCC3 is a novel target for the treatment of pancreatic cancer." Advances in biological regulation 73 (2019): 100634.
Pubmed: 31053501 DOI: 10.1016/j.jbior.2019.04.004

In the context of breast cancer, this work emphasizes the significance of ABCC3 transporters in drug resistance to chemotherapy. Furthermore, our findings imply that regular chemotherapy in conjunction with combinatorial inhibition of these transporters can lessen therapy-induced resistance in breast cancer.
Balaji, Sai A., et al. "Role of the drug transporter ABCC3 in breast cancer chemoresistance." PloS one 11.5 (2016): e0155013.
Pubmed: 27171227 DOI: 10.1371/journal.pone.0155013