mProX™ Human ABCC2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-0922-KX1556	Magic™ Human ABCC2 in Vitro Drug screening assay	Human	Caco-2, MDCK, HEK293, CHO, membrane vesicles, and various transfected cells lines	Activity Assay (High throughput screening assays; Large scale single dose, duplicate profiling; IC50 profiling; Ki determination assay; Substrate determination assay )

Product Information

Target Protein

ABCC2

Target Family

ABC Transporter

Target Protein Species

Human

Host Cell Type

CNE2; CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

Cancer Research; Metabolic Research

Related Diseases

Dubin-Johnson Syndrome; Pseudoxanthoma Elasticum

Gene ID

1244

UniProt ID

Q92887

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Multidrug resistance protein 2 (MRP2) is a protein that is produced by the ABCC2 gene. This protein is a member of a family of multidrug resistance proteins that are involved in the exocytosis of drugs. For instance, MRP2 participates in drug metabolism by removing specific medications from tissues and organs. Additionally, MRP2 moves bilirubin-a chemical produced by the liver-from liver cells into bile, the digestive fluid produced by the liver. The breakdown of aged red blood cells produces bilirubin, which has an orange-yellow color. MRP2 is mostly present in the outer membrane that envelops liver cells; traces of it are also present in the kidneys, gut, and placenta. The customized ABCC2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Ashley

The ABCC2 cell line design is well-optimized, and the provided documentation is comprehensive. Apr 12 2021

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chat Stephanie

I would recommend this product to anyone who wants to research ABCC2. Nov 01 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Immunocytochemical staining of ABCC2 expression.

ABCC2 expression was stained immunocytochemically in three (×200) cells using the SP technique, DAB staining, and hematoxylin counterstaining. (a) Blank control; (b) CDDP-resistant CNE2-CDDP cells; (c) parental cell line CNE2; and (d) CDDP-5Fu-resistant CNE2-CDDP-5Fu cells. ABCC2 protein is found in the cytoplasm in the black arrow and in the nucleus in the red arrow.

Ref: Xie, Si Ming, et al. "Association of ABCC2 and CDDP-resistance in two sublines resistant to CDDP derived from a human nasopharyngeal carcinoma cell line." Journal of oncology 2010 (2010).

Pubmed: 20628484

DOI: 10.1155/2010/915046

Research Highlights

The body has a variety of broad specificity transporters in its excretory organs and several epithelial blood-tissue barriers to aid in the removal of ambient toxins and metabolic waste products.
van der Schoor, Lori WE, et al. "New insights in the biology of ABC transporters ABCC2 and ABCC3: impact on drug disposition." Expert opinion on drug metabolism & toxicology 11.2 (2015): 273-293.
Pubmed: 25380746 DOI: 10.1517/17425255.2015.981152

It has been discovered that certain human malignancies overexpress multidrug resistance-associated protein 2 (ABCC2), an ATP-binding cassette multidrug resistance transporter. According to this study, cisplatin (DDP)-resistant A549 cells (A549/DDP) also had increased expression of ABCC2.
Chen, Yun, et al. "Increased ABCC2 expression predicts cisplatin resistance in non-small cell lung cancer." Cell Biochemistry and Function 39.2 (2021): 277-286.
Pubmed: 32815556 DOI: 10.1002/cbf.3577