mProX™ Human ABCC1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-0922-KX1557	Magic™ Human ABCC1 in Vitro Drug screening assay	Human	Caco-2, MDCK, HEK293, CHO, membrane vesicles, and various transfected cells lines	Activity Assay (High throughput screening assays; Large scale single dose, duplicate profiling; IC50 profiling; Ki determination assay; Substrate determination assay )

Product Information

Target Protein

ABCC1

Target Family

ABC Transporter

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

Auditory and Otology Research

Related Diseases

Deafness; Autosomal Dominant Non-Syndromic Sensorineural Deafness Type Dfna

Gene ID

4363

UniProt ID

P33527

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Numerous chemotherapeutic drugs and conjugates of glutathione, glucuronide, and sulfate are among the many organic anions that human ABCC1 transports. By facilitating leukotriene-mediated inflammatory responses, defending against oxidative stress by extruding GSSG from cells, and shielding human bodies against toxic endobiotics and xenobiotics, ABCC1 is a multifunctional GS-X pump. These activities also establish ABCC1's crucial role in MDR in the chemotherapy of various cancers. Several in vivo and in vitro investigations have shown that ABCC1 downregulation causes various anticancer medications, which are ABCC1 substrates, to become chemosensitized. This suggests that human ABCC1 is a viable target for reversing MDR in a variety of cancer types. The customized ABCC1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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This ABCC1 KD cell line has saved us time and resources. Our team is excited to delve deeper into our investigations with this versatile tool. Jul 13 2022

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I think this mouse ABCC1 cell line is better than many similar products already on the market. Jun 04 2020

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FAQ

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Published Data

Fig.1 Effect of mutations on MRP1/ABCC1 expression HEK293 and CHO-K1 cells.

HEK293 and CHO-K1 cells were transiently transfected with vector control or wild-type, Cys43Ser, Thr73Ile, Arg723Gln, and Arg1058Gln mutant MRP1/ABCC1 to examine the effect of mutations on MRP1/ABCC1 expression. The RNAs were then prepared for quantitative real-time RT-PCR analysis of MRP1/ABCC1 mRNA level.

Ref: Yin, Ji-Ye, et al. "Characterization and analyses of multidrug resistance-associated protein 1 (MRP1/ABCC1) polymorphisms in Chinese population." Pharmacogenetics and genomics 19.3 (2009): 206.

Pubmed: 19214144

DOI: 10.1097/FPC.0b013e328323f680

Research Highlights

One important regulating mechanism that reduces cell-intrinsic STING activation and improves STING-dependent autoimmune illness is ABCC1-mediated cGAMP export.
Maltbaek, Joanna H., et al. "ABCC1 transporter exports the immunostimulatory cyclic dinucleotide cGAMP." Immunity 55.10 (2022): 1799-1812.
Pubmed: 36070769 DOI: 10.1016/j.immuni.2022.08.006

Different tissues respond differently to different steroids due to the varied transport propensities of both endogenous and exogenous glucocorticoids by ABCB1 and ABCC1 transporters.
Devine, Kerri, et al. "The ATP-binding cassette proteins ABCB1 and ABCC1 as modulators of glucocorticoid action." Nature Reviews Endocrinology 19.2 (2023): 112-124.
Pubmed: 36221036 DOI: 10.1038/s41574-022-00745-9