mProX™ Human ABCB11 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-0922-KX1560	Magic™ Human ABCB11 in Vitro Drug screening assay	Human	Caco-2, MDCK, HEK293, CHO, membrane vesicles, and various transfected cells lines	Activity Assay (High throughput screening assays; Large scale single dose, duplicate profiling; IC50 profiling; Ki determination assay; Substrate determination assay )

Product Information

Target Protein

ABCB11

Target Family

ABC Transporter

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

Digestive and Renal Research; Metabolic Research

Related Diseases

Cholestasis

Gene ID

8647

UniProt ID

O95342

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The ABCB11 gene in humans encodes the protein ABCB11. BSEP, or sPgp, is the ABC transporter that is produced by the ABCB11 gene. The ATP-binding cassette (ABC) transporter superfamily includes this membrane-associated protein. Different substances are transported across extracellular and intracellular membranes by ABC proteins. There are seven different subfamilies of ABC genes. This protein belongs to the subfamily known as MDR/TAP. The multidrug resistance subfamily includes certain MDR/TAP members. The movement of taurocholate and other cholate conjugates from hepatocytes, or liver cells, to the bile is carried out by this specific protein. In humans, bile flow and production are mostly determined by the activity of this transporter. The customized ABCB11 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Donna

I've used the ABCB11 cell line in multiple assays, and each time, it has exceeded my expectations. Jun 17 2022

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chat Cynthia

The human ABCB11 overexpression cell line has not disappointed. It consistently delivers accurate results, making my work much more impactful. Mar 11 2023

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FAQ

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Published Data

Fig.1 Human bile salts exporter ABCB11.

In the presence or absence of 40 μM taurocholic and two inhibitors (GC glycocholic, TC taurocholic, TUDC tauroursodesoxycholic, Rif rifampicin, and Glib glibenclamide), the relative ATPase activity of ABCB11 is examined.

Ref: Wang, Liang, et al. "Cryo-EM structure of human bile salts exporter ABCB11." Cell Research 30.7 (2020): 623-625.

Pubmed: 32203132

DOI: 10.1038/s41422-020-0302-0

Research Highlights

In biliary secretion, the bile salt export pump (BSEP) is crucial. The disease known as progressive familial intrahepatic cholestasis type 2 (PFIC2), which manifests as severe jaundice and liver failure, is caused by mutations in the ABCB11 gene, which codes for BSEP.
Imagawa, Kazuo, et al. "Clinical phenotype and molecular analysis of a homozygous ABCB11 mutation responsible for progressive infantile cholestasis." Journal of Human Genetics 63.5 (2018): 569-577.
Pubmed: 29507376 DOI: 10.1038/s10038-018-0431-1

Bile acids are a class of physiological surfactants generated from cholesterol that are necessary for the digestion of fats and fat-soluble vitamins.
Wang, Liang, et al. "Structures of human bile acid exporter ABCB11 reveal a transport mechanism facilitated by two tandem substrate-binding pockets." Cell Research 32.5 (2022): 501-504.
Pubmed: 35043010 DOI: 10.1038/s41422-021-00611-9