mProX™ Human ABCB1 Stable Cell Line

Product Information

Target Protein

ABCB1

Target Family

ABC Transporter

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

Inflammation Research

Related Diseases

Inflammatory Bowel Disease; Colchicine Resistance

Gene ID

5243

UniProt ID

P08183

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

In humans, the ABCB1 gene encodes the glycoprotein P-gp. Within the MDR/TAP subfamily, P-gp is a well-characterized ABC-transporter. Certain pharmaceutical medications become less effective pharmacokinetically when xenobiotics are regularly excreted back into the gastrointestinal lumen by P-gp. Furthermore, a significant amount of P-gp is expressed by certain cancer cells, which amplifies the previously mentioned effect and makes the tumors resistant to several drugs. P-gp is widely expressed and distributed. It is involved in the intestinal epithelium's function of pumping foreign substances back into the intestinal lumen, in the liver's ability to pump substances into bile ducts, in the kidney's proximal tubule cells' function of pumping substances into urine filtrate, and in the capillary endothelial cells that make up the blood-brain and blood-testis barriers' function of returning substances to the capillaries. The customized ABCB1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Matthew

The ABCB1 cell line was an indispensable tool in my research. The cell line's stability and reproducibility were outstanding, allowing for reliable results across multiple experiments. Mar 27 2022

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chat Michelle

The ABCB1 overexpression cell line has played a pivotal role in our journey of discovery. Jul 14 2020

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FAQ

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Published Data

Fig.1 Increased ABCB1 expression is associated with resistance to olaparib.

Western blot analysis of whole cell lysates from the specified cell lines was performed for ABCB1. Tubulin functioned as a brake on loading.

Ref: Lombard, Alan P., et al. "Overexpressed ABCB1 induces olaparib-taxane cross-resistance in advanced prostate cancer." Translational oncology 12.7 (2019): 871-878.

Pubmed: 31075528

DOI: 10.1016/j.tranon.2019.04.007

Research Highlights

This review's objective was to assess how ABCB1 polymorphisms affect breast cancer treatment outcomes, which will be crucial for customizing anticancer therapy in the future.
Tulsyan, Sonam, Rama Devi Mittal, and Balraj Mittal. "The effect of ABCB1 polymorphisms on the outcome of breast cancer treatment." Pharmacogenomics and personalized medicine (2016): 47-58.
Pubmed: 27175090 DOI: 10.2147/PGPM.S86672

The ATP-binding cassette transporter ABCB1 (P-glycoprotein/P-gp) is well-known for its correlation with multidrug resistance in cancer cells. Driven by ATP hydrolysis, it eliminates a variety of structurally different molecules.
Chufan, Eduardo E., Hong-May Sim, and Suresh V. Ambudkar. "Molecular basis of the polyspecificity of P-glycoprotein (ABCB1): recent biochemical and structural studies." Advances in cancer research 125 (2015): 71-96.
Pubmed: 25640267 DOI: 10.1016/bs.acr.2014.10.003