Vasopressin GPCR Assays
Background of Vasopressin Receptors
Vasopressin receptors belong to the family of G protein-coupled receptors (GPCR). The Vasopressin receptors mediate various biological functions, such as vasoconstriction, antidiuresis, and adrenocorticotropic hormone (ACTH) release.
Fig.1. Physiological effects of vasopressin. (Demiselle, 2020)
Distributions and Functions of Vasopressin Receptors
AVPR1A is found in high density on vascular smooth muscle and induces various biological functions, such as vasoconstriction, facilitating thrombosis, and stimulating VEGF secretion. AVPR1B located in the hypothalamus controls ACTH secretion, while AVPR1B located in the pancreas is involved in insulin release. The expression of AVPR2V2 can only be found on the basolateral membrane of collecting duct cells in the kidney, for the regulation of water excretion.
Subtypes and Mechanisms of Vasopressin Receptors
There are three subtypes of vasopressin receptors, including arginine vasopressin receptor 1A (AVPR1A), Arginine vasopressin receptor 1B (AVPR1B), and Arginine vasopressin receptor 2 (AVPR2).
| Receptor | Gene | Mechanism | Agonists | Antagonists |
| Arginine vasopressin receptor 1A | AVPR1A |
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| Arginine vasopressin receptor 1B | AVPR1B |
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| Arginine vasopressin receptor 2 | AVPR2 |
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Assay List of Vasopressin Receptors
Creative Biolabs can provide a range of assays of vasopressin receptors. You can choose the assay in the list or contact us for more information:
Published Data
| Paper Title | Vasopressin receptor subtypes and renal sodium transport |
| Journal | Vitamins and Hormones |
| Published | 2020 |
| Abstract | In mammals, three V receptor subtypes have been identified in the kidney. Three different receptor subtypes, V2, V1a, and V1b, trigger vasopressin, a hormone synthesized in the hypothalamus. Stimulation of V2 receptors can modulate urine osmolarity. Stimulation of V1a receptors inhibits renal sodium reabsorption and induces urinary sodium excretion. Stimulation of V1b receptors induces potassium secretion in the final part of the distal segment and the initial part of the collecting duct. In this paper, we describe the interaction of vasopressin with V receptor subtypes to better understand the role of these systems and their regulation in facilitating the identification of other systemic components and mechanisms. |
| Result |
Physiologically, it is important to simultaneously characterize the characteristics and capabilities of each Na+ transport system in the renal tubule and their roles under conditions of different functional states of the organism. We already know that the involvement of various subtypes of V receptors plays a key role in stabilizing the physicochemical parameters of the human internal environment and water-salt homeostasis. Increased renal Na+ excretion is involved in osmotic homeostasis mechanisms when serum Na+ concentrations are elevated and arginine vasopressin (AVP) levels are elevated. This suggests that V1aR and V2R are activated under hypernatremia. Furthermore, the mode by which AVP regulates water and Na+ transport is physiologically appropriate under conditions of dehydration. Vasopressin is a unique physiologically active substance that stimulates different subtypes of V receptors and maintains extracellular fluid volume and osmolarity. It may be developed as a novel therapeutic agent to promote water-salt homeostasis.
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Fig.2. Localization of V-receptors and sodium transport systems in the kidney. (Natochin & Golosova, 2020)
References
- Demiselle, J.; et al. Vasopressin and its analogues in shock states: a review. Annals of intensive care. 2020, 10(1): 1-7.
- Natochin, Y.V.; Golosova, D.V. Vasopressin receptor subtypes and renal sodium transport. Vitamins and Hormones. 2020, 113: 239-258.
