Neuropeptide Y GPCR Assays

The neuropeptide Y receptor gene repository, phylogeny and comparative expression in allotetraploid common carp

Scientific Reports

2022

The NPY family receptors are specific receptors for the endogenous appetite-stimulating factor neuropeptide Y, which lays the physiological foundation for the transmembrane transport of its related peptides. NPY exists widely in various species and affects the neuroendocrine system, regulating appetite and stress feedback. In order to better understand the evolutionary footprint and functional information of NPYR genes, this study conducted a detailed survey of NPYR genes in 10 representative species, and the NPYR gene abundance, structure, conserved gene sequence, and phylogenetic relationship of allo/autotetraploid and diploid species were analyzed. The researchers speculate that the abundance, distribution, and expression profiles of NPYR among different species can provide us with more comprehensive information on the evolutionary route and functional exploration of NPYR genes, and help us gain a deeper understanding of the evolutionary journey of organisms.

In this study, the researchers selected more than ten representative species expressing NPYR, and explored the evolutionary footprint and tissue distribution of NPYR in different organisms. The focus of the study is the investigation of the NPYR gene in allotetraploid carp. Experiments revealed two isoforms of twelve PNYR genes in allotetraploid carp by multiple alignment, phylogenetic relationship, syntenic analysis, and structural analysis. The analysis results show that the number of NPYR genes in tetraploid species is twice that of diploid species, but the distribution of NPYR genes in autotetraploid and diploid species in the same habitat is not strictly 2:1. suggest that ancient whole-genome duplication events may have been followed by gene loss.

This study clarified the evolutionary fate of the NPYR gene under species-specific whole-genome duplication events in teleosts, especially the evolutionary process of allotetraploid carp after the fourth round of whole-genome duplication. Genome-wide research and expression profile comparison of NPYR may provide new insights and theoretical support for exploring the evolutionary path and specific gene functions of more organisms in the future.

Fig.2. Phylogenetic tree of NPYR in vertebrates. (Zou, 2022)