mProX™ Human WNK3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX527	Magic™ Human WNK3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;H2009;H460

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research

Related Diseases

Motor Stereotypies; Sleep Apnea

Gene ID

Human:65267

UniProt ID

Human:Q9BYP7

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The applications of WNK3 include its role in osmosensing and hydrostatic pressure sensing. WNK3 is regulated by chloride, osmotic stress, and hydrostatic pressure, and it functions as an allosteric inhibitor of WNK kinases. Crystallography studies have shown that WNK3 is stabilized by a conserved water network and a cluster of charged residues. Mutations in these residues can enhance WNK3 activity. WNK3 has also been implicated in testis cell pyroptosis and the pathogenesis of Sertoli cell-only syndrome. Additionally, WNK3 interacts with Cullin 3 and is involved in cerebral ischemia-reperfusion injury. High-throughput screening has identified WNK isoform-specific inhibitors, which may have therapeutic potential for hypertension and cancer.

Protocols

Please visit our protocols page.

Customer Reviews

There are currently no Customer reviews or questions for mProX™ Human WNK3 Stable Cell Line (S01YF-1023-PY132). Click the button above to contact us or submit your feedback about this product.

FAQ

chat Cameron Jones (Verified Customer)

How does WNK3 maintain neuronal excitability? May 12 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

WNK3 kinase maintains neuronal excitability by regulating inwardly rectifying K+ conductance in neurons, impacting sensorimotor gating. May 12 2023

chat Alex Williams (Verified Customer)

Can WNK3 inhibition enhance antitumor immunity? Jul 16 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

Inhibition of WNK3 suppresses PD-L1 expression on tumor cells and activates T-cell function, suggesting its potential in cancer immunotherapy. Jul 16 2021

Published Data

Fig.1 Knocking down WNK3 has a notable impact on the JNK/C-JUN pathway.

The impact of WNK3 depletion on the phosphorylation status of established PD-L1 transcription regulators, including JNK, c-JUN, ERK, P38, AKT, p65, and STAT3, was evaluated through immunoblotting of whole-cell lysates obtained from the H2009 and H460 cell lines. Distinguished alterations in protein expression are indicated by arrowheads, while β-Actin served as the reference for loading control.

Ref: Yoon, Hyun Ju, et al. "WNK3 inhibition elicits antitumor immunity by suppressing PD-L1 expression on tumor cells and activating T-cell function." Experimental & Molecular Medicine 54.11 (2022): 1913-1926.

Pubmed: 36357569

DOI: 10.1038/s12276-022-00876-z

Research Highlights

R Teixeira, Liliana. et al. "Water and chloride as allosteric inhibitors in WNK kinase osmosensing." bioRxiv : the preprint server for biology, 2023.
In this study, it was found that both chloride and osmotic stress play a role in regulating the autophosphorylation and activity of the kinase domains of WNK1 and WNK3. Additionally, crystallography of the unphosphorylated WNK1 kinase domain (WNK1/S382A, WNK1/SA) showed a presence of conserved waters in the active site. New research has revealed through crystallography that the application of PEG400 to WNK1/SA crystals in the P1 space group leads to de-dimerization and a change in space group to P2. This result suggests potential implications for WNK1 and its role in cellular signaling.
R Teixeira, Liliana. et al. "Water and chloride as allosteric inhibitors in WNK kinase osmosensing." bioRxiv : the preprint server for biology, 2023.
Pubmed: 37693587 DOI: 10.1101/2023.08.29.555411

M Humphreys, John. et al. "Hydrostatic Pressure Sensing by WNK kinases." Molecular biology of the cell, 2023.
This study has shown that the WNK kinases 1 and 3 serve as direct osmosensors, aligning with their known function in regulating cell volume. There is also evidence suggesting that these WNK kinases may be impacted by hydrostatic pressure. This effect was observed in cells cultured with N-terminal SNPs. Their findings support a potential link between hydrostatic pressure and the function of WNK kinases in cellular regulation.
M Humphreys, John. et al. "Hydrostatic Pressure Sensing by WNK kinases." Molecular biology of the cell, 2023.
Pubmed: 37585288 DOI: 10.1091/mbc.E23-03-0113