mProX™ Human TYRO3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX522	Magic™ Human TYRO3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;SCC-25;MGH-U3

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research

Related Diseases

Lymphocytic Choriomeningitis; Zika Fever

Gene ID

Human:7301

UniProt ID

Human:Q06418

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

TYRO3, a member of the TAM (TYRO3, AXL, Mer) family of receptor tyrosine kinases, has been implicated in various physiological and pathological processes, including inflammation, adipocyte function, and autoimmune disorders. In the context of acute lung injury (ALI), TYRO3 has been shown to play a role in cell proliferation, apoptosis, and inflammatory response in pulmonary epithelial cells. In the study of human body fat distribution genes, TYRO3 was identified as a key driver gene that may regulate fat distribution by altering adipocyte function. In rheumatoid arthritis (RA), TYRO3 was found to contribute to the pathogenic phenotypes of fibroblast-like synoviocytes and disturb immune cell balance, suggesting it as a potential therapeutic target for RA. Additionally, TYRO3 has been recognized as a therapeutic target at the interface of cancer and immunity, as it promotes cell survival, metastasis, and resistance to therapy in cancer cells, while also suppressing antitumor immunity. Inhibition of TYRO3 has shown promise in preclinical and clinical studies as a potential cancer treatment strategy.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Morgan Garcia (Verified Customer)

What is the role of TYRO3 in colorectal cancer? Mar 22 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

TYRO3 has been identified as a target gene in colorectal cancer, where its regulation is crucial for cancer cell proliferation, migration, invasion, and glycolysis. Mar 22 2021

chat Skyler Garcia (Verified Customer)

How does TYRO3 influence bone homeostasis? Jun 21 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

TYRO3, along with MERTK, plays a significant role in regulating bone homeostasis, with implications in cancer and other diseases. Jun 21 2021

Published Data

Fig.1 Investigating the consequences of Tyro3 depletion on cancer cell viability post staurosporine-triggered apoptosis, and evaluating the influence of supplemented TAM ligand on these outcomes.

The impact of Tyro3 knockdown on the survival of cancer cells subjected to staurosporine-induced apoptosis, along with the influence of additional TAM ligand, was assessed through MTS assays conducted on MGH-U3 and SCC-25 cells. After transfection with either control siRNA (siControl) or Tyro3 siRNA #2 (siTyro3) for 48 hours at 50 nM, the cells were exposed to staurosporine (STS; 0.1 μM) in the presence of either ProS1 (7.5 nM) or Gas6 (5.7 nM) for the final 20 hours. The resulting data, representing mean ± SEM, were analyzed using Student's two-tailed t-test, revealing significant differences (***p<0.001, **p<0.01, *p<0.05) or non-significant results (ns) compared to the respective siControl for each treatment, as indicated by lines (n = 3 separate experiments).

Ref: Al Kafri, Nour, and Sassan Hafizi. "Identification of signalling pathways activated by Tyro3 that promote cell survival, proliferation and invasiveness in human cancer cells." Biochemistry and Biophysics Reports 28 (2021): 101111.

Pubmed: 34471705

DOI: 10.1016/j.bbrep.2021.101111

Research Highlights

Cheng, Yujing. et al. "The role of the Gas6/TAM signal pathway in the LPS-induced pulmonary epithelial cells injury." Molecular immunology, 2023.
Acute lung injury (ALI) is a condition that causes sudden inflammation in the respiratory system. One key factor in this process is the interaction between growth arrest-specific 6 (Gas6) and tyrosine kinases of the Tyro3, Axl, Mer (TAM) family. This interaction plays a significant role in various physiological and pathological processes, such as inflammation. The current study focused on understanding the specific mechanism of the Gas6/TAM pathway in lipopolysaccharide (LPS)-induced injury of pulmonary epithelial cells (BEAS-2B cells).
Cheng, Yujing. et al. "The role of the Gas6/TAM signal pathway in the LPS-induced pulmonary epithelial cells injury." Molecular immunology, 2023.
Pubmed: 37820442 DOI: 10.1016/j.molimm.2023.10.001

N Reed, Jordan. et al. "Systems genetics analysis of human body fat distribution genes identifies Wnt signaling and mitochondrial activity in adipocytes." bioRxiv : the preprint server for biology, 2023.
Excess fat in the abdominal region is a well-known risk factor for cardio-metabolic disease, and its link to sexual dimorphism further highlights the need for further research in this area. One way of approximating the relative distribution of abdominal and lower-body subcutaneous fat is through the waist-to-hip ratio adjusted for body mass index (WHRadjBMI). Previous genome-wide association studies have revealed 346 loci near 495 genes that are associated with WHRadjBMI. While the functions of many of these genes in fat distribution remain unknown, several are expressed in adipose tissue and may play a role in this process. To further elucidate the relationship between these genes and WHRadjBMI, a team aimed to use a systems genetics approach to identify novel sex- and depot-specific drivers of this risk factor.
N Reed, Jordan. et al. "Systems genetics analysis of human body fat distribution genes identifies Wnt signaling and mitochondrial activity in adipocytes." bioRxiv : the preprint server for biology, 2023.
Pubmed: 37732278 DOI: 10.1101/2023.09.06.556534