mProX™ Human TSSK1B Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX515	Magic™ Human TSSK1 in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1

Target Classification

Kinase Cell Lines

Target Research Area

Orphan Receptor Research

Related Diseases

Stiff Skin Syndrome; Ciliary Dyskinesia, Primary, 19

Gene ID

Human:83942

UniProt ID

Human:Q9BXA7

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

TSSK1B is a testis-specific serine kinase that has been studied in the context of sexual maturity, male infertility, and male contraception. In terms of sexual maturity, TSSK1B is one of the candidate genes that are under positive selection and play a role in testicular maturation. It is highly expressed in the testes and may be involved in enhancing boar reproductive traits. In the field of male infertility, mutations in TSSK1B have been associated with asthenoteratozoospermia, azoospermia, and teratozoospermia. These mutations may be a cause of undiagnosed cases of male infertility and should be considered in molecular diagnostics. Additionally, TSSK1B has been investigated as a potential target for male contraception, and computational methods have been used to study its binding affinity to inhibitors. Overall, TSSK1B is an important gene involved in testicular development, male fertility, and potential contraceptive strategies.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Casey Smith (Verified Customer)

What is the role of TSSK1B in male contraception? Jun 16 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

TSSK1B is a validated target for male contraception, with studies establishing a quantitative structure-activity relationship for inhibitors. Jun 16 2023

chat Alex Williams (Verified Customer)

How does TSSK1B expression relate to male infertility? Oct 19 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

TSSK1B is specifically expressed in testes and its expression patterns in yak and cattle-yak hybrids suggest a role in male fertility. Oct 19 2020

Published Data

Fig.1 Correlation analysis between sperm chromatin compaction, various semen parameters and transcript levels of TH2B associated genes.

A heatmap depicting the Spearman correlation analysis was generated, illustrating the relationships between sperm chromatin compaction metrics (%AB+ and %CMA3+), various semen characteristics (motility, concentration, viability), and the expression levels of genes associated with TH2B (CREM, CDYL, PRKAG2, CATSPERB, TSGA10, TSSK1B). Statistical significance levels were indicated as p* for <0.05, p** for <0.01, p*** for <0.001, and p**** for <0.0001. This analysis served to elucidate the interconnectedness of chromatin compaction in sperm with semen parameters and specific gene transcripts.

Ref: Patankar, Aniket, et al. "Epigenetic landscape of testis specific histone H2B variant and its influence on sperm function." Clinical epigenetics 13.1 (2021): 1-18.

Pubmed: 33933143

DOI: 10.1186/s13148-021-01088-4

Research Highlights

Li, Xiu-Ling. et al. "Integrated testis transcriptome and whole genome analysis of sexual maturity in Large White and Tongcheng pigs." Reproduction in domestic animals = Zuchthygiene, 2023.
The study investigated the genetic basis and key genes related to sexual maturity through measuring serum testosterone levels and analyzing testis tissue sections of Large White (LW) and Tongcheng (TC) boars at an immature age. By using RNA-seq, differentially expressed genes (DEGs) in testis development were identified in both breeds. Additionally, the selection signatures of both breeds were analyzed to determine which DEGs underwent positive selection. Results showed that TC pigs had higher serum testosterone levels and larger cross-sectional areas and perimeters of seminiferous tubules compared to LW pigs. A total of 5068 DEGs were identified based on filtering criteria of q value <0.05 and |log2FC|>1.
Li, Xiu-Ling. et al. "Integrated testis transcriptome and whole genome analysis of sexual maturity in Large White and Tongcheng pigs." Reproduction in domestic animals = Zuchthygiene, 2023.
Pubmed: 37151112 DOI: 10.1111/rda.14373

Liu, Xiaorong. et al. "QSAR via multisite λ-dynamics in the orphaned TSSK1B kinase." Protein science : a publication of the Protein Society, 2023.
Multisite λ-dynamics (MSλD) presents an innovative approach to computing relative binding free energies between ligands and their target receptors, offering a robust tool for structure-based drug design by accommodating diverse molecules with multiple functional groups at various sites around a common core. In this investigation, MSλD is employed to assess the relative binding free energies of 1296 inhibitors binding to testis-specific serine kinase 1B (TSSK1B), a validated target for male contraception. MSλD proves computationally efficient in comparison to conventional methods like free energy perturbation or thermodynamic integration. The study delves into the interplay of ligand modifications at distinct sites, establishing a quantitative structure-activity relationship (QSAR) and pinpointing a ligand site with potential for enhanced binding affinity through the incorporation of polar groups.
Liu, Xiaorong. et al. "QSAR via multisite λ-dynamics in the orphaned TSSK1B kinase." Protein science : a publication of the Protein Society, 2023.
Pubmed: 36906820 DOI: 10.1002/pro.4623