mProX™ Human TNIK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX509	Magic™ Human TNIK(ZC2) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1

Target Classification

Kinase Cell Lines

Target Research Area

Metabolic Research

Related Diseases

Intellectual Developmental Disorder, Autosomal Recessive 54; Autosomal Recessive Non-Syndromic Intellectual Disability

Gene ID

Human:23043

UniProt ID

Human:Q9UKE5

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

TNIK (Traf2- and NCK-interacting protein kinase) has various applications in different fields. In the field of metabolism, TNIK has been identified as a regulator of glucose and lipid metabolism in obesity and type 2 diabetes. Studies have shown that TNIK governs lipid and glucose homeostasis in both Drosophila and mice, and Tnik knockout mice exhibit improved lipid profiles and enhanced glucose and lipid handling. In the field of cancer research, TNIK has been found to play a role in colorectal cancer cell metastasis and papillary thyroid carcinoma. Loss of LKB1, a gene associated with colorectal cancer, leads to upregulation of TNIK, which promotes cell motility and tumor metastasis. In papillary thyroid carcinoma, TNIK expression is elevated and may contribute to carcinogenesis and progression. Additionally, TNIK has been implicated in ovarian cancer, where it is targeted by miR-5590-3p to inhibit cell proliferation and invasion through the Wnt/Œ≤-catenin signaling pathway. Finally, in the field of cardiovascular disease, TNIK deficiency in B cells does not affect atherosclerosis. These studies highlight the diverse roles of TNIK in different biological processes and diseases.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Cameron Williams (Verified Customer)

What is the significance of TNIK in cancer research? Mar 30 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

TNIK has been identified as a protein involved in several cancers, with studies showing its potential as a target for kinase inhibitors in cancer therapy. Mar 30 2020

chat Alex Jones (Verified Customer)

How does TNIK affect Wnt signaling in colon cancer? Dec 07 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

TNIK plays a crucial role in the Wnt signaling pathway, a main driver of cell growth in colorectal cancer (CRC), and its inhibition can suppress CRC growth. Dec 07 2021

Published Data

Fig.1 In a dose-dependent manner, the transcription activity of the Tcf-reporter TOPFlash was abolished by the expression of all TNIK kinase mutants.

β-catenin/TCF-driven transcription was abrogated by the expression of TNIK kinase mutants (depicted in the second, third, and fourth panels), while an increase in β-catenin/TCF-driven transcription was specifically induced by the overexpression of WT TNIK (as shown in the last panel) in Ls174T CRCs. TOPFlash activity was denoted by black bars, and FOPFlash activity was represented by green bars at 24 hours post transfection of expression vectors, with error bars indicating standard deviation across three independent experiments. Expression of Flag-tagged WT and mutant TNIK proteins was detected using M2 Flag antisera in the Western blot analysis (bottom panel). Significant differences were observed with *P-value<0.05 and **P-value<0.01.

Ref: Mahmoudi, Tokameh, et al. "The kinase TNIK is an essential activator of Wnt target genes." The EMBO journal 28.21 (2009): 3329-3340.

Pubmed: 19816403

DOI: 10.1038/emboj.2009.285

Research Highlights

C Phung Pham, T. et al. "TNIK is a conserved regulator of glucose and lipid metabolism in obesity." Science advances, 2023.
According to recent research, obesity and type 2 diabetes (T2D) are significant health concerns with unresolved treatment requirements. The Traf2- and NCK-interacting protein kinase (TNIK) has been identified as a gene associated with obesity and T2D, but its specific functions have yet to be determined. Findings indicate that TNIK plays a crucial role in regulating the balance of lipids and glucose in the body, highlighting its potential as a therapeutic target for these conditions.
C Phung Pham, T. et al. "TNIK is a conserved regulator of glucose and lipid metabolism in obesity." Science advances, 2023.
Pubmed: 37556547 DOI: 10.1126/sciadv.adf7119

Hu, Guanghui. et al. "LKB1 loss promotes colorectal cancer cell metastasis through regulating TNIK expression and actin cytoskeleton remodeling." Molecular carcinogenesis, 2023.
The study focused on the role of liver kinase B1 (LKB1), also known as STK11, in colorectal cancer (CRC). LKB1 is a major gene responsible for Peutz-Jeghers syndrome (PJS), which is associated with approximately 5%-6% of CRC cases. Through the use of CRISPR-Cas9, LKB1 knockout (KO) CRC cell lines were generated and their behavior was studied in vitro and in vivo. Results showed that LKB1 deficiency enhanced migration and metastasis of CRC cells, and this was mediated by upregulation of TRAF2 and NCK-interacting protein kinase (TNIK), which was repressed by LKB1's kinase activity. Further experiments revealed that TNIK promoted cytoskeleton remodeling through its interaction with ARHGAP29. These findings suggest that the LKB1-TNIK axis may play a critical role in CRC progression.
Hu, Guanghui. et al. "LKB1 loss promotes colorectal cancer cell metastasis through regulating TNIK expression and actin cytoskeleton remodeling." Molecular carcinogenesis, 2023.
Pubmed: 37449799 DOI: 10.1002/mc.23606