mProX™ Human TNFSF9 Stable Cell Line

Xie, Long. et al. "RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma." PloS one, 2023.
Receptor activity modifying protein 1 (RAMP1) plays a crucial role in facilitating the localization of calcitonin-like receptor (CLR) to the plasma membrane. However, its role in osteosarcoma (OS) remains unclear. A recent study evaluated the expression of RAMP1 and its prognostic value across various cancers, with a particular focus on tumor immune infiltration. This analysis utilized the GSE39058 and TARGET datasets and found that high RAMP1 expression was associated with poor prognosis in OS patients (p<0.05). Additionally, differential gene expression and protein-protein interaction networks were analyzed, and gene set enrichment analysis was performed. These findings suggested that RAMP1 may play a critical role in the tumor microenvironment, as its expression was also validated in OS cell lines using quantitative real-time PCR. Notably, low RAMP1 expression was linked to an abundance of CD4+ memory-activated T cells, while high expression correlated with a high proportion of gamma-delta T cells. Furthermore, differential gene expression analysis from the TARGET dataset revealed an enrichment of olfactory transduction pathways (normalized enrichment scores [NES] = 1.6998, p < 0.0001). Interestingly, RAMP1 expression was also found to be negatively correlated with CD44 expression but positively correlated with TNFSF9 expression. Further analysis showed significantly higher RAMP1 gene expression in OS cells compared to a normal osteoblast cell line, hFOB1.19. These findings suggest that RAMP1 may serve as a prognostic biomarker and potential therapeutic target in OS.
Xie, Long. et al. "RAMP1 as a novel prognostic biomarker in pan-cancer and osteosarcoma." PloS one, 2023.
Pubmed: 37796823   DOI: 10.1371/journal.pone.0292452

Xu, Haozhe. et al. "Elevated extracellular calcium ions accelerate the proliferation and migration of HepG2 cells and decrease cisplatin sensitivity." Journal of biomedical research, 2023.
Hepatoblastoma, the most common type of liver cancer in children, has been the focus of numerous studies. In one particular study, a team of researchers observed that the addition of calcium ions to the culturing medium of HepG2, a cell line derived from hepatoblastoma, resulted in a reduction of cell clumping. Further investigations revealed that this calcium signal plays a crucial role in the development of tumors. The team's findings suggest that calcium ions induce morphological changes and promote cell proliferation and migration through the activation of FAK, protein kinase B, and p38 mitogen-activated protein kinase signaling pathways.
Xu, Haozhe. et al. "Elevated extracellular calcium ions accelerate the proliferation and migration of HepG2 cells and decrease cisplatin sensitivity." Journal of biomedical research, 2023.
Pubmed: 37750331   DOI: 10.7555/JBR.37.20230067