mProX™ Human TMEM175 Stable Cell Line

Product Information

Target Family

Other Targets

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;RAW264.7

Target Classification

Other Targets Drug Discovery Assays and Products

Target Research Area

CNS Research

Related Diseases

Meier-Gorlin Syndrome 3; Parkinson Disease, Late-Onset

Gene ID

Human:84286

UniProt ID

Human:Q9BSA9

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

TMEM175 is a lysosomal ion channel that has been associated with neurological diseases. It plays a role in maintaining lysosomal acidity and assisting in the digestion of abnormal proteins and organelles. Abnormal expression of TMEM175 has been observed in amyotrophic lateral sclerosis (ALS), a neurodegenerative disease. In a study, six ALS risk genes, including TMEM175, were identified, and the dysregulation of TMEM175 in multiple tissues was found. TMEM175 has also been implicated in Parkinson's disease (PD), with a positive correlation and causal effect observed between PD and ALS. Several single-nucleotide polymorphisms and genes, including TMEM175, have been associated with both diseases. Additionally, TMEM175 has been studied in the context of neonatal hypoxic-ischemic brain injury, where its downregulation was found to contribute to impairment of the autophagy-lysosome pathway. Overall, TMEM175 shows promise as a therapeutic target for neurodegenerative diseases, and further research is needed to explore its potential in the treatment of these conditions.

Protocols

Please visit our protocols page.

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FAQ

chat Alex Davis (Verified Customer)

What is the role of TMEM175 in lysosomal function? Dec 03 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

TMEM175 is a lysosomal potassium channel that maintains lysosomal pH homeostasis, crucial for cellular degradation processes and implicated in diseases like Parkinson's. Dec 03 2022

chat Casey Jones (Verified Customer)

How does TMEM175 contribute to neurological diseases? Sep 19 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

TMEM175 dysfunction is linked to neuronal injury in conditions like cerebral ischemia-reperfusion, suggesting its role in neuroprotection and disease pathogenesis. Sep 19 2020

Published Data

Fig.1 The elimination of I_KEL was achieved through the knockout of mTMEM175, with the subsequent transfection o.htmEM175 cDNA into the mouse knockout cells resulting in the rescue and enhancement of I_KEL, serving as an additional control measure.

Voltage-clamped recordings of lysosomal currents were obtained from three experimental conditions: a control group, a knockout (KO) group, and a knockout cell that underwent transfection wit.htmEM175 (KO .htmEM175). The average current amplitudes at +100 mV were measured. Recordings were conducted using a ramp protocol (from -100 mV to +100 mV in 1 s) with 150 mM NMDG in the pipettes and 150 mM K+ (or NMDG, as specified) in the bath, showcasing the utilization of these components in the experiments.

Ref: Cang, Chunlei, et al. "TMEM175 is an organelle K+ channel regulating lysosomal function." Cell 162.5 (2015): 1101-1112.

Pubmed: 26317472

DOI: 10.1016/j.cell.2015.08.002

Research Highlights

Ma, Yanni. et al. "Abnormal Brain Protein Abundance and Cross-tissue mRNA Expression in Amyotrophic Lateral Sclerosis." Molecular neurobiology, 2023.
In this study, the researchers conducted a two-stage proteome-wide association study (PWAS) using ALS genome-wide association study (GWAS) data and two distinct human brain protein quantitative trait loci (pQTL) datasets to identify additional causative genes for amyotrophic lateral sclerosis (ALS). Candidate genes were prioritized using Mendelian randomization (MR) and Bayesian colocalization analysis. The researchers also verified the expression of these genes in multiple tissues, including lower motor neurons, skeletal muscle, and whole blood. Six ALS risk genes (SCFD1, SARM1, TMEM175, BCS1L, WIPI2, and DHRS11) were identified during the PWAS discovery phase, and SARM1 and BCS1L were later confirmed during the validation phase. The MR and colocalization results showed significant associations with ALS risk.
Ma, Yanni. et al. "Abnormal Brain Protein Abundance and Cross-tissue mRNA Expression in Amyotrophic Lateral Sclerosis." Molecular neurobiology, 2023.
Pubmed: 37639066 DOI: 10.1007/s12035-023-03587-2

Bazzone, Andre. et al. "A Comparative Study on the Lysosomal Cation Channel TMEM175 Using Automated Whole-Cell Patch-Clamp, Lysosomal Patch-Clamp, and Solid Supported Membrane-Based Electrophysiology: Functional Characterization and High-Throughput Screening Assay Development." International journal of molecular sciences, 2023.
The protein TMEM175, known to be involved in Parkinson has shown potential as a target for drug development. While automated patch-clamp has limitations for studying lysosomal channels, a new method called solid supported membrane-based electrophysiology (SSME) allows for both direct access to and high-throughput recording of lysosomes. Utilizing this technique, the team was able to stimulate ion translocation through TMEM175 using a concentration gradient with a resting potential of 0 mV, resulting in concentration-dependent K+/H+ exchange and subsequent TMEM175 currents.
Bazzone, Andre. et al. "A Comparative Study on the Lysosomal Cation Channel TMEM175 Using Automated Whole-Cell Patch-Clamp, Lysosomal Patch-Clamp, and Solid Supported Membrane-Based Electrophysiology: Functional Characterization and High-Throughput Screening Assay Development." International journal of molecular sciences, 2023.
Pubmed: 37628970 DOI: 10.3390/ijms241612788