mProX™ Human TLR2 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Immune Checkpoint Cell Lines
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Published Data
Fig.1 IL-8 release from HEK 293 cells is induced by HMGB1 when expressed in E. coli or CHO cells, with TLR2 overexpression, while it is not elicited by TLR4 overexpression or vector.
At 90% confluence, HEK 293 cells that had been overexpressed with either TLR2, TLR4, or vector alone (pcDNA3) were plated into 96-well culture plates and subjected to stimulation with recombinant HMGB1 produced in either E. coli or mammalian CHO cells. Positive controls, employing LPS (ligand for TLR4, 100 ng/mL) or Pam2CSK4 (ligand for TLR2, 1 ng/mL), were utilized for an 18-hour duration. Subsequently, the conditioned media were subjected to an ELISA assay to measure IL-8 release. The data were represented as mean ± SEM and were derived from 3-6 independent experiments. Significance was denoted as *P < 0.05 compared to the unstimulated control.
Ref: Yu, Man, et al. "HMGB1 signals through toll-like receptor (TLR) 4 and TLR2." Shock 26.2 (2006): 174-179.
Pubmed: 16878026
DOI: 10.1097/01.shk.0000225404.51320.82
Research Highlights
Bang, Sunghee. et al. "A Cardiolipin from Muribaculum intestinale Induces Antigen-Specific Cytokine Responses." Journal of the American Chemical Society, 2023.
A systematic phenotypic screen was conducted on the mouse gut microbiome to identify metabolites with an immunomodulatory effect. This study identified a total of 60 compounds. These findings have potential implications for understanding the role of the gut microbiome in regulating immune response and may contribute to the development of new therapies for immune-related disorders. The methodology utilized in this study may serve as a valuable tool for future research in this area.
Bang, Sunghee. et al. "A Cardiolipin from Muribaculum intestinale Induces Antigen-Specific Cytokine Responses." Journal of the American Chemical Society, 2023.
Pubmed:
37871232
DOI:
10.1021/jacs.3c09734
Zhang, Yongtao. et al. "Weizmannia coagulans Extracellular Proteins Reduce Skin Acne by Inhibiting Pathogenic Bacteria and Regulating TLR2/TRAF6-Mediated NF-κB and MAPKs Signaling Pathways." Probiotics and antimicrobial proteins, 2023.
In this research, the anti-acne effects and mechanism of Weizmannia coagulans (W. coagulans) were evaluated. The focus was on the extracellular proteins (YTCY-EPs) from the YTCY strain of W. coagulans, which are composed primarily of peptides. These peptides were found to possess antimicrobial and antioxidative properties, with a strong impact on Cutibacterium acnes and Staphylococcus aureus. In vitro experiments demonstrated the ability of YTCY-EPs to reduce active oxygen, inhibit inflammatory pathways, and regulate the expression of inflammatory genes, leading to a decrease in inflammation and acne development. In animal testing, a reduction in inflammatory factors and improvement in acne symptoms was observed after treatment with YTCY-EPs. These findings suggest that YTCY-EPs could potentially be utilized as a natural ingredient in cosmetics for the treatment of acne.
Zhang, Yongtao. et al. "Weizmannia coagulans Extracellular Proteins Reduce Skin Acne by Inhibiting Pathogenic Bacteria and Regulating TLR2/TRAF6-Mediated NF-κB and MAPKs Signaling Pathways." Probiotics and antimicrobial proteins, 2023.
Pubmed:
37870674
DOI:
10.1007/s12602-023-10175-2
