mProX™ Human SIGLEC6 Stable Cell Line

Thi Nguyen, Thuy. et al. "Complement Regulatory Protein CD46 Manifests a Unique Role in Promoting the Migration of Bladder Cancer Cells." Chonnam medical journal, 2023.
CD46 is a membrane-bound complement regulatory protein (mCRP) that plays a crucial role in regulating the complement system to protect host cells from damage. It is highly expressed in various cancers, including bladder cancers, and acts as a receptor for cancer therapeutic viruses. In this study, the authors reveal a new function of CD46 as a progressor of bladder cancer cells. Using DNA microarray analysis, the authors identified several target genes, including C3α, MGP, AFAP-AS1, FDCSP, MAMDC2, GABRP, TGFBI, CYP24A1, SIGLEC6, MT1E, and cytokeratins, which were confirmed through quantitative RT-PCR and Western blot analyses. Further investigations showed that CD46 also enhances the migratory potential of bladder cancer cells by regulating MGP and KRT13, both implicated in cell migration and cancer metastasis. Overall, this study highlights the overexpression of CD46 in bladder cancers and its unique role in promoting cancer cell migration. Future studies are required to elucidate the mechanism of CD46 action and its application in cancer therapeutics.
Thi Nguyen, Thuy. et al. "Complement Regulatory Protein CD46 Manifests a Unique Role in Promoting the Migration of Bladder Cancer Cells." Chonnam medical journal, 2023.
Pubmed: 37840671   DOI: 10.4068/cmj.2023.59.3.160

Lubin, Ruth. et al. "Human Dendritic Cell Enrichment and Their Activation of T Cells." Current protocols, 2023.
Dendritic cells (DCs) play a critical role in orchestrating the immune response to various threats throughout the body. These cells, which bridge the gap between innate and adaptive immunity, have historically been classified as conventional (cDC) and plasmacytoid (pDC). However, recent research has shown that cDCs consist of various subsets with distinct functions. To fully examine the diversity of cDCs in both healthy and pathological conditions, a robust experimental pipeline is necessary, including an efficient cell enrichment protocol. Unfortunately, many commercially available DC enrichment kits were developed before the discovery of these distinct subsets. In this study, two approaches for enriching human blood DCs or specific subsets are described, along with an in vitro protocol for examining DC stimulation of naïve T cells. These methods have been developed to overcome the challenges encountered when enriching human DC subsets. Basic Protocol 1 outlines a method for enriching pDC, Axl Siglec6-DC (AS-DC), cDC1, DC2, DC3, monocytes, and HLA-1-internal cells.
Lubin, Ruth. et al. "Human Dendritic Cell Enrichment and Their Activation of T Cells." Current protocols, 2023.
Pubmed: 37610279   DOI: 10.1002/cpz1.873