mProX™ Human RPS6KA6 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX483	Magic™ Human RSK4(RPS6KA6) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;DLD-1;HT29;SW480

Target Classification

Kinase Cell Lines

Target Research Area

Pain and Addiction Research

Related Diseases

Non-Syndromic X-Linked Intellectual Disability 77; Coffin-Lowry Syndrome

Gene ID

Human:27330

UniProt ID

Human:Q9UK32

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

RPS6KA6 (Ribosomal S6 kinase 4) has been implicated in various biological processes and diseases. In ovarian cancer, RPS6KA6 promoter methylation was observed, but it was not found to regulate its expression in ovarian cancer. In donkeys, RPS6KA6 was identified as a candidate gene associated with body size traits. In hepatocellular carcinoma, RPS6KA6 was part of an integrated RNA network and was found to be related to the prognosis of patients. In keratoconus, RPS6KA6 was identified as one of the candidate genes involved in the genetic spectrum of the disease. In different cancers, the expression of RPS6KA6 isoforms was found to be associated with clinical features, but the contradictory roles of RPS6KA6 in cancers cannot be explained solely by differential isoform expression. Further research is needed to understand the context-dependent function of RPS6KA6.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Morgan Brown (Verified Customer)

How does RPS6KA6 affect thyroid cancer? Feb 08 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

RPS6KA6 is regulated by FOXP2 and plays a role in thyroid cancer cell proliferation and apoptosis, possibly via the PI3K/AKT pathway. Feb 08 2023

chat Skyler Smith (Verified Customer)

What is the significance of RPS6KA6 in acute myeloid leukemia? Feb 27 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

Down-regulation of RPS6KA6 in acute myeloid leukemia may lead to increased activity of the ERK/MPAK pathway, exacerbating leukemogenesis. Feb 27 2020

Published Data

Fig.1 A reduction in cell viability is observed when mutant TMC3 co-occurs with the knockdown of RPS6KA6 through siRNA.

A knockdown of RPS6KA6 was achieved via siRNA in cells harboring either mutant TMC3 (SW480 cells) or wild-type TMC3 (DLD-1 and HT29 cells). Cell viability was assessed over a five-day period using the Cell Counting Kit-8 (CCK-8). On the fifth day, a significant reduction in viable SW480 cells was observed in the presence of the mutant protein.

Ref: Zhou, Yuan, et al. "Integrated multi-omics data analyses for exploring the co-occurring and mutually exclusive gene alteration events in colorectal cancer." Human Mutation 41.9 (2020): 1588-1599.

Pubmed: 32485022

DOI: 10.1002/humu.24059

Research Highlights

Arechavaleta-Velasco, Fabian. et al. "Ribosomal S6 kinase 4 (RSK4) tumor suppressor gene promoter methylation status in ovarian cancer." Molecular biology reports, 2023.
The authors of this study had previously reported lower levels of RSK4 mRNA and protein in malignant ovarian tumors when compared to normal and benign ovarian tissues. They also noted a significant inverse correlation between advanced stages of ovarian cancer and RSK4 mRNA levels. However, the mechanisms behind the reduced expression of RSK4 in ovarian cancer had not been investigated. Therefore, this study aimed to explore whether RSK4 promoter methylation in ovarian cancer tissues was responsible for its low expression. The impact of reactivating RSK4 expression was also examined in ovarian cancer cell lines.
Arechavaleta-Velasco, Fabian. et al. "Ribosomal S6 kinase 4 (RSK4) tumor suppressor gene promoter methylation status in ovarian cancer." Molecular biology reports, 2023.
Pubmed: 37402066 DOI: 10.1007/s11033-023-08609-9

Song, Shuang. et al. "Genome-wide association study to identify SNPs and candidate genes associated with body size traits in donkeys." Frontiers in genetics, 2023.
The Yangyuan donkey is a domestic animal breed mainly found in the northwest region of Hebei Province. Its body shape is a crucial production indicator that can accurately reflect the donkey's growth and is closely linked to valuable economic traits. As a key factor in breeding selection, body size traits play a significant role in monitoring animal growth and assessing selection response. The utilization of molecular markers, which are genetically linked to body size traits, holds promise for expediting the animal breeding process.
Song, Shuang. et al. "Genome-wide association study to identify SNPs and candidate genes associated with body size traits in donkeys." Frontiers in genetics, 2023.
Pubmed: 36926587 DOI: 10.3389/fgene.2023.1112377