mProX™ Human ROR2 Stable Cell Line

Product Information

Target Family

Other Targets

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;MDA-MB-231;MCF-7

Target Classification

Other Targets Drug Discovery Assays and Products

Target Research Area

CNS Research

Related Diseases

Robinow Syndrome, Autosomal Recessive 1; Brachydactyly, Type B1

Gene ID

Human:4920

UniProt ID

Human:Q01974

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

ROR2, a Wnt receptor, plays a role in the neuronal Wnt5a/Ca2+ signaling pathway, regulating synaptic and neuronal function. It can activate key signaling events in neurons, including kinase activation, increased calcium levels, and enhanced trafficking of synaptic components. In hepatocellular carcinoma (HCC), the expression of ROR2 is associated with tumor differentiation and prognosis. An electrochemical immunosensor has been developed to detect ROR2, which shows good sensitivity and selectivity. In tetralogy of Fallot (TOF), ROR2 deficiency is implicated in the down-regulation of specific genes and pathways involved in cardiac development. Hyperglycemia increases the secretion of SCO-spondin and Wnt5a, which regulate ependymal cell beating and glucose sensing in the cerebrospinal fluid. Understanding ROR2 signaling is crucial for comprehending its role in various biological processes.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Alex Jones (Verified Customer)

How does ROR2 interact with Wnt signaling? Mar 09 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

ROR2 can interact with Wnt signaling pathways, such as non-canonical Wnt signaling, influencing developmental processes and potentially cancer progression. Mar 09 2020

chat Alex Jones (Verified Customer)

What are the implications of ROR2 mutations in diseases like Robinow syndrome? Jan 31 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

Mutations in ROR2 can lead to conditions like Robinow syndrome, with the phenotype determined by the degree of protein retention/degradation and membrane localization. Jan 31 2020

Published Data

Fig.1 ROR2 was subjected to knockdown using the MDA-MB-231 cell line, while ROR2 overexpression was induced in the MCF-7 cell line.

The expression of ROR2 was analyzed by Western blotting in MDA-MB-231 and MCF-7 cells, which had been transfected with siROR2 and pLenti-ROR2 plasmids.

Ref: Guo, Muhong, et al. "ROR2 knockdown suppresses breast cancer growth through PI3K/ATK signaling." Aging (Albany NY) 12.13 (2020): 13115.

Pubmed: 32614787

DOI: 10.18632/aging.103400

Research Highlights

Riquelme, Raul. et al. "ROR2 homodimerization is sufficient to activate a neuronal Wnt/calcium signaling pathway." The Journal of biological chemistry, 2023.
In the mature central nervous system (CNS), Wnt signaling assumes a pivotal role by overseeing the movement of NMDA-type glutamate receptors and the intrinsic characteristics of neurons. Within this context, the Wnt receptor ROR2 emerges as a vital element in the neuronal Wnt5a/Ca2+ signaling pathway that governs synaptic and neuronal operation. Despite being labeled as a pseudokinase, the exact mechanism of ROR2's downstream signaling upon ligand binding has sparked debate. Recent findings indicate that chemically induced homodimerization of ROR2 can effectively reproduce crucial signaling events following receptor activation in neurons, such as the activation of PKC and JNK kinases, the elevation of somatic and dendritic Ca2+ levels, and the enhanced trafficking of NMDARs to synapses. Furthermore, ROR2 homodimerization induces receptor phosphorylation on Tyr residues, implying an active kinase domain. These results signify that ROR2 possesses the capacity to signal independently of Frizzled receptors, impacting the trafficking of a vital synaptic component and suggesting that homodimerization can override structural constraints that render the tyrosine kinase inactive. Gaining a deeper understanding of ROR2 signaling holds significant implications for unraveling the regulation of synaptic and neuronal function in mature animal brain processes.
Riquelme, Raul. et al. "ROR2 homodimerization is sufficient to activate a neuronal Wnt/calcium signaling pathway." The Journal of biological chemistry, 2023.
Pubmed: 37832874 DOI: 10.1016/j.jbc.2023.105350

Wakizaka, Kazuki. et al. "Expression of Wnt5a and ROR2, Components of the Noncanonical Wnt-Signaling Pathway, is Associated with Tumor Differentiation in Hepatocellular Carcinoma." Annals of surgical oncology, 2023.
In this study, the role of receptor tyrosine kinase-like orphan receptor 2 (ROR2) in the association between the noncanonical Wnt signaling pathway and hepatocellular carcinoma (HCC) was examined. The research focused on the expression of ROR2, a receptor associated with the key ligand of the noncanonical Wnt pathway, Wnt5a. The significance of ROR2 expression in the development of HCC was investigated, as its contribution to the noncanonical Wnt pathway in carcinogenesis remains uncertain.
Wakizaka, Kazuki. et al. "Expression of Wnt5a and ROR2, Components of the Noncanonical Wnt-Signaling Pathway, is Associated with Tumor Differentiation in Hepatocellular Carcinoma." Annals of surgical oncology, 2023.
Pubmed: 37814183 DOI: 10.1245/s10434-023-14402-6