mProX™ Human ROCK2 Stable Cell Line

Salah Fayed, Hend. et al. "Selective ROCK Inhibitor Enhances Blood Flow Recovery after Hindlimb Ischemia." International journal of molecular sciences, 2023.
The impairment in microvascular network formation can delay the restoration of blood flow after acute limb ischemia. A high-content screen of a GSK-published kinase inhibitor library identified a set of ROCK inhibitor hits that enhance endothelial network formation. Further evaluation through kinase activity profiling against a panel of 224 protein kinases revealed the high selectivity of two indazole-based ROCK inhibitor hits for ROCK1 and ROCK2 isoforms compared to other ROCK inhibitors. One of these compounds, GSK429286, showed promising results and was chosen for subsequent studies. It was found to be ten times more potent in enhancing endothelial tube formation than the classic ROCK inhibitor Fasudil. Inhibition of ROCK1 via RNAi was shown to have a similar angiogenic effect as GSK429286. In an organotypic angiogenesis co-culture assay, GSK429286 was observed to form a dense vascular network with thicker endothelial tubes. When administered to mice (10 mg/kg i.p.) for seven days after hindlimb ischemia induction, GSK429286 significantly increased blood flow recovery, as assessed by laser speckle contrast imaging. Histological analysis revealed that GSK429286 also promoted the growth of new microvessels in the affected muscles. These findings suggest that selective ROCK inhibitors have pro-angiogenic properties in vitro and hold therapeutic potential for restoring blood flow in limb ischemia.
Salah Fayed, Hend. et al. "Selective ROCK Inhibitor Enhances Blood Flow Recovery after Hindlimb Ischemia." International journal of molecular sciences, 2023.
Pubmed: 37833857   DOI: 10.3390/ijms241914410

Jin, Yu-Qiu. et al. "Mechanism of Zhenwu Decoction in improving renal inflammatory injury in mice with DN of spleen-kidney Yang deficiency syndrome by regulating ROCK/IKK/NF-κB pathway." Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2023.
To explore the impact and underlying mechanism of Zhenwu Decoction on diabetic nephropathy (DN) in mice with spleen-kidney Yang deficiency syndrome via the Rho-associated coiled-coil kinase (ROCK)/IκB kinase (IKK)/nuclear factor-κB (NF-κB) pathway, a study utilized 7-week-old db/db male mice and db/m male mice. DN was induced using Dahuang Decoction and hydrocortisone, followed by random grouping into model, high-dose, medium-dose, low-dose Zhenwu Decoction, and irbesartan groups. After eight weeks of intervention, various parameters were assessed. The results indicated significant improvements in renal function and pathology in DN mice treated with Zhenwu Decoction, potentially linked to the suppression of the ROCK/IKK/NF-κB pathway-mediated inflammatory response in the kidneys.
Jin, Yu-Qiu. et al. "Mechanism of Zhenwu Decoction in improving renal inflammatory injury in mice with DN of spleen-kidney Yang deficiency syndrome by regulating ROCK/IKK/NF-κB pathway." Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2023.
Pubmed: 37802846   DOI: 10.19540/j.cnki.cjcmm.20230512.402