mProX™ Human RARG Stable Cell Line

Product Information

Target Protein

RARG

Target Family

Retinoic Acid Receptor

Target Protein Species

Human

Host Cell Type

HCC; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Autoimmune Research; Cancer Research; Inflammation Research

Related Diseases

Embryonal Carcinoma; Exfoliative Ichthyosis

Gene ID

5916

UniProt ID

P13631

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The nuclear receptor known as retinoic acid receptor gamma (RAR-γ) is encoded by the RARG gene. Adapalene specifically targets the retinoic acid receptor beta and retinoic acid receptor gamma; the effects of adapalene are primarily due to its agonism of the gamma subtype. Mice lacking RARγ show growth deficits. Anteriorization of the cervical and thoracic vertebrae is one of the axial skeleton's abnormalities caused by the absence of RARγ, which controls hindbrain and axial patterning. The development of healthy alveoli and alveolar elastic fibers in the lung depends on RARγ. Male sterility is caused by genetic ablation of RARγ, which is also linked to keratinization of glandular epithelia and squamous metaplasia of the prostate gland and seminal vesicles. The customized RARG stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Carol

I purchased the RARG cell line from Creative Biolabs and the provided documentation was comprehensive and helped me optimize my assay conditions. Mar 09 2023

chat Verified Customer

chat Kevin

I've been using the human RARG cell line for my studies, and it has been an excellent choice. Jan 13 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 The expression levels of RARγ and E-cadherin in HCC cell lines.

RT-PCR was used to assess RARγ and E-cadherin expression in the designated cell lines. The mRNA levels of E-cadherin and RARγ in HCC cell lines are correlated using dot plots. The dotted line illustrates the inverse relationship between E-cadherin and RARγ at the mRNA levels.

Ref: Gan, Wen-Juan, et al. "RARγ-induced E-cadherin downregulation promotes hepatocellular carcinoma invasion and metastasis." Journal of Experimental & Clinical Cancer Research 35.1 (2016): 1-11.

Pubmed: 27756432

DOI: 10.1186/s13046-016-0441-9

Research Highlights

More than 50% of juvenile cancer treatment protocols include anthracyclines; nevertheless, anthracycline-induced cardiotoxicity (ACT) limits the clinical use of anthracyclines by causing asymptomatic cardiac dysfunction and congestive heart failure in up to 57% and 16% of patients, respectively.
Aminkeng, Folefac, et al. "A coding variant in RARG confers susceptibility to anthracycline-induced cardiotoxicity in childhood cancer." Nature genetics 47.9 (2015): 1079-1084.
Pubmed: 26237429 DOI: 10.1038/ng.3374

Within the nuclear receptor superfamily, retinoic acid receptor α (RARα) and retinoic acid receptor β (RARβ) are 90% homologous with retinoic acid receptor γ (RARγ). Although acute promyelocytic leukemia (APL) is known to be associated with RARA rearrangements, RARG rearrangements can also mimic this type of leukemia.
Conserva, Maria Rosa, et al. "RARG gene dysregulation in acute myeloid leukemia." Frontiers in Molecular Biosciences 6 (2019): 114.
Pubmed: 31709264 DOI: 10.3389/fmolb.2019.00114